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核酸功能化纳米探针检测水体中痕量Pb^(2+)的研究

Nucleic acid functionalized nanoprobs for the detection of trace Pb^(2+)in aqueous media
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摘要 铅离子(Pb^(2+))属于三大重金属污染物之一,是一种严重危害环境和人体健康的重金属元素。基于此,本研究建立一种简便、经济的水体中Pb^(2+)的检测方法。将纳米金颗粒(AuNP)作为荧光团标记脱氧核糖核酸酶(DNAzyme)的载体和猝灭剂,两者形成AuNP DNAzyme纳米探针。利用DNAzyme对目标物Pb^(2+)进行特异性识别,Pb^(2+)催化DNAzyme底物链裂解,实现荧光信号恢复。基于荧光信号的变化,实现了Pb^(2+)在5~125 nmol/L范围内的线性检测,并且Pb^(2+)的检出限为3.41 nmol/L。此外,该传感器对Pb^(2+)有较好的抗干扰性能,对Pb^(2+)加标的河水样品进行检测,回收率为95.90%~100.83%。表明该传感器在环境监测领域具有广阔的应用前景。 Lead ion(Pb^(2+)),one of the three major heavy metal pollutants,can seriously harm the environment and human health.Herein,we developed a simple and economical method for the detection of Pb^(2+)in water using AuNP-DNAzyme nanoprobes,which were constructed with gold nanoparticles(AuNP)as fluorophore labeled deoxyribonuclease(DNAzyme)carrier and quench agent.Through the specific recognition of Pb^(2+)by DNAzyme and subsequent Pb^(2+)-catalyzed fragmentation of substrate chain,the fluorescence signal could be restored for analytical purpose.As a result,our method showed high sensitivity for Pb^(2+),with linear detection at 5-125 nmol/L range and calibration limit of 3.41 nmol/L.In addition,our sensor demonstrated excellent anti-interference performance to Pb^(2+)and proved to be effective for the analysis of Pb^(2+)added river water samples,leading to 95.90%-100.83%recovery.Our research suggests the sensor with AuNP-DNAzyme nanoprobe can potentially have broad application in the field of environmental monitoring.
作者 张勤 姜涵 李向玲 宋天顺 谢婧婧 ZHANG Qin;JIANG Han;LI Xiangling;SONG Tianshun;XIE Jingjing(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China)
出处 《生物加工过程》 CAS 2024年第3期302-308,共7页 Chinese Journal of Bioprocess Engineering
基金 国家自然科学基金面上基金(22078149) 江苏省高等学校自然科学面上项目(20KJB150020)。
关键词 铅离子 DNAZYME 纳米金颗粒 AuNP DNAzyme纳米探针 重金属污染 lead ion DNAzyme gold nanoparticles AuNP-DNAzyme nanoprobe heavy metal pollution
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