蛋白质组学技术及其在氟中毒研究中的应用

Proteomics technology and its application in the study of fluorosis

氟在自然界中广泛存在,是机体必需微量元素之一。摄入少量氟对机体骨骼及牙齿有保护作用,但长期过量摄入氟会造成机体慢性氟中毒,对机体造成骨相及非骨相损伤,严重威胁着动物的健康。随着蛋白质组学技术的发展及其在氟中毒研究中的应用,人们对氟的骨相及非骨相毒性的分子机制研究越来越深入。笔者主要介绍了包括质谱分析、双向电泳、高效液相色谱分析、免疫共沉淀、同位素标记相对和绝对定量及蛋白质芯片等蛋白质组学技术的原理及特点,并概述了这些蛋白质组学技术在氟的骨相和非骨相毒性研究中的应用,旨在为进一步探究氟中毒的致病机制提供思路和见解。

Fluorine exists widely in nature and is one of the essential trace elements in the body.A small amount of fluorine has protective effects on bones and teeth,but long-term excessive intake of fluorine can cause chronic fluorosis and damage to bone tissue and non-bone tissues of the body,which seriously threatens the health of animals.With the development of proteomics technology and its application in the study of fluorosis,the molecular mechanism of bone and non-bone toxicity of fluorine has been studied more and more deeply.The author mainly introduced the principles and characteristics of proteomic technologies,including mass spectrometry(MS),two-dimensional electrophoresis(2-DE),high performance liquid chromatography(HPLC),co-immunoprecipitation(Co-IP),isobaric tags for relative and absolute quantitation(iTRAQ) and protein microarray,and summarized the application and research of these proteomic technologies in the bone tissue and non-bone tissue toxicity caused by fluorine,in order to provide ideas and insights for further exploring the pathogenic mechanism of fluorosis.