总评归一法结合响应面法优化滋阴清骨丸提取工艺

Extraction process optimization for preparation of Ziyin Qinggu Pills by overall desirability and response surface methodology

目的优选滋阴清骨丸的最佳提取工艺。方法以溶剂量、提取时间、浸泡时间为影响因素,以知母皂苷BⅡ、芍药苷、毛蕊异黄酮苷、甘草苷各成分含量及干浸膏得率的总评归一值(OD值)为评价指标,采用中心组合设计-响应面法优选滋阴清骨丸的最佳提取工艺。结果滋阴清骨丸最佳提取工艺为:提取时间115 min,浸泡时间26 min,溶剂量10倍,提取2次。结论优化的提取工艺合理、稳定、可靠,可为滋阴清骨丸的提取工艺及进一步成型工艺提供依据。

Objective To optimize the extraction process of Ziyin Qinggu Pills.Methods The solvent amount,extraction time and soaking time were taken as the influencing factors,and the overall desirability(OD)of the contents of timosaponin BⅡ,paeoniflorin,calycosin 7-O-glucoside,liquiritin and the dry extract yield were taken as the evaluation indexes,so that the optimal extraction process of Ziyin Qinggu Pills was optimally selected by the centralized composite design-response surface method.Results The optimal extraction process of Ziyin Qinggu Pills was as follows:extraction time of 115 min,soaking time of 26 min,solvent volume of 10 times,and extraction twice.Conclusion The optimal extraction process is reasonable,stable and reliable,which can provide the basis and foundation for the extraction process and further molding process of Ziyin Qinggu Pills.