茯苓酸对宫颈癌细胞增殖、侵袭及凋亡的影响

Effect of pachymic acid on proliferation,invasion and apoptosis of cervical cancer cells

目的探讨茯苓酸对宫颈癌细胞增殖、侵袭及凋亡的影响,并初步探讨其调控机制。方法体外培养人宫颈癌Hela细胞,分别以5.0、10.0、20.0μmol/L的茯苓酸处理细胞,未进行任何处理的Hela细胞作为对照组。克隆形成实验与MTT实验测定不同浓度茯苓酸对Hela细胞增殖的影响;Transwell实验检测不同浓度茯苓酸对细胞侵袭能力的影响;流式细胞仪检测不同浓度茯苓酸对细胞凋亡情况的影响;Western blot方法检测不同浓度茯苓酸对细胞Wnt、Cyclin D1与基质金属蛋白酶(MMP)-2表达的影响。结果与对照组相比,5.0、10.0、20.0μmol/L的茯苓酸均能明显抑制宫颈癌Hela细胞的增殖能力与侵袭能力,同时诱导Hela细胞凋亡;不同浓度的茯苓酸均能抑制Hela细胞Wnt、Cyclin D1与MMP-2蛋白的表达水平,且呈浓度依赖性。结论茯苓酸能够抑制人宫颈癌Hela细胞增殖与侵袭,并促进凋亡。

Objective To investigate the effects and its regulatory mechanism of pachymic acid on the proliferation,invasion and apoptosis of cervical cancer cells.Methods Human cervical cancer Hela cells were cultured in vitro and treated with 5.0,10.0 and 20.0μmol/L pachymic acid,respectively,and untreated Hela cells served as the control group.The effect of different concentrations of pachymic acid on cell proliferation was detected by MTT assay and clonal formation assay.The effect of different concentrations of pachymic acid on cell invasion was detected by transwell assay.The effect of different concentrations of pachymic acid on cell apoptosis was detected by flow cytometry.The expressions of Wnt,Cyclin D1 and matrix metalloproteinase(MMP)-2 were detected by Western blot.Results Compared with the control group,5.0,10.0 and 20.0μmol/L pachymic acid could significantly inhibit the proliferation and invasion ability of Hela cells,and induce the apoptosis of Hela cells.The expression levels of Wnt,Cyclin D1 and MMP-2 in Hela cells were inhibited by different concentrations of pachymic acid in a concentration-dependent manner.Conclusion Pachymic acid can inhibit the proliferation and invasion of human cervical cancer Hela cells and induce apoptosis.