血小板裂解液对Wistar大鼠创伤性膝软骨损伤中Runx2、SOX9表达的影响

Effect of platelet lysate on the expression of Runx2 and SOX9 in cartilage injury of Wistar rats

目的探讨血小板裂解液(PL)在修复Wistar大鼠创伤性膝软骨损伤中对Runx2、SOX9表达的影响。方法将30只Wistar大鼠随机分成对照组、生理盐水组和血小板裂解液组,每组10只。HE染色和番红O-固绿染色观察各组大鼠膝关节软骨情况,并进行Mankin评分;免疫组织化学染色检测大鼠膝关节软骨Runx2、SOX9的表达;Western blot检测大鼠膝关节软骨Runx2、SOX9的表达。结果经过PL注射后,创伤性膝软骨损伤大鼠软骨组织损伤明显减轻,软骨Mankin评分明显降低,膝关节软骨组织Runx2、SOX9阳性表达细胞数明显降低,膝关节软骨组织Runx2、SOX9的蛋白表达明显降低(P<0.01)。结论血小板裂解液可促进Wistar大鼠软骨组织结构性恢复,降低软骨损伤后Runx2、SOX9表达水平。

Objective To investigate the effect of platelet lysate(PL)on the expressions of Runx2 and SOX9 in the repair of traumatic cartilage injury in Wistar rats.Methods Thirty Wistar rats were randomly divided into control group,saline group and platelet lysate group,with 10 rats in each group.HE staining and safranin O-fast green staining were used to observe the knee cartilage of rats in each group,and Mankin score was used.Immunohistochemical staining was used to detect the expressions of Runx2 and SOX9 in knee cartilage of rats,and Western blot was used to detect the expressions of Runx2 and SOX9 in knee cartilage of rats.Results After PL injection,the injury of cartilage tissue in rats with traumatic knee cartilage injury was significantly alleviated,the Mankin score of cartilage was significantly reduced,the number of cells of Runx2 and SOX9 positive expression in knee cartilage tissue was significantly reduced,and the protein expressions of Runx2 and SOX9 in knee cartilage tissue were significantly reduced(P<0.01).Conclusion Platelet lysate can promote the structural recovery of cartilage tissue in Wistar rats and reduce the expression of Runx2 and SOX9 after cartilage injury.