摘要
目的探究常山酮(HF)对大鼠心肌缺血/再灌注心律失常的作用。方法30只Wistar大鼠随机分为假手术组(Sham)、模型组(Model)与HF组,每组10只,结扎冠状动脉左前降支建立大鼠心肌缺血/再灌注模型,假手术组大鼠只穿线不结扎。同时HF组大鼠舌下静脉注射给药常山酮(5 mg/kg),Sham和Model组大鼠给予等容量的生理盐水。分别在缺血期、再灌注期,每5 min测量1次心率。TTC法检测心肌损伤面积;TUNEL染色检测心肌细胞凋亡;ELISA检测大鼠血清SOD活性和MDA含量;试剂盒检测大鼠心肌组织Ca2+水平。Western blot检测大鼠心肌组织Nrf2和HO-1蛋白的表达。结果HF降低大鼠缺血期心率,上调再灌注期心率;HF减少MIRI大鼠心肌梗死面积,降低心肌细胞凋亡率,提高血清SOD活性,下调血清MDA水平,下调心肌组织中的Ca2+含量,激活Nrf2/HO-1信号通路。结论常山酮通过激活Nrf2/HO-1信号通路减轻心肌缺血再灌注所致心肌细胞氧化损伤,改善心肌缺血/再灌注心律失常。
Objective To explore the effect of halofuginone(HF)on myocardial ischemia-reperfusion arrhythmias in rats.Methods Thirty Wistar rats were randomly divided into Sham group,Model group and HF group,with 10 rats in each group.The left anterior descending coronary artery was ligation to establish the rat myocardial ischemia/reperfusion model.The rats in sham group were only ligation without ligation.At the same time,the rats in HF group were given sublingual intravenous injection(5 mg/kg),and the rats in Sham and Model groups were given equal volume of normal saline.The heart rate was measured every 5 minutes during ischemia and reperfusion.The area of myocardial injury was detected by TTC.The apoptosis of cardiomyocyte was detected by TUNEL staining.Activity of SOD and content of MDA in serum were detected by ELISA.The levels of Ca~(2+)in rat myocardial tissue was detected.The expressions of Nrf2 and HO-1 in myocardial tissue of rats were detected by Western blot.Results HF decreased the heart rate during ischemia and increased the heart rate during reperfusion.HF decreased the myocardial infarction size,decreased the apoptosis rate of cardiomyocytes,increased activity of SOD in serum,down-regulated level of MDA in serum,down-regulated content of Ca~(2+)in myocardial tissue,and activated Nrf2/HO-1 signaling pathway in MIRI rats.Conclusion HF could alleviate myocardial ischemia/reperfusion induced-myocardial oxidative damage and improve myocardial ischemia/reperfusion arrhythmia by activating Nrf2/HO-1 signaling pathway.
作者
王彦新
陈立涛
刘振华
吴腾飞
WANG Yan-xin;CHEN Li-tao;LIU Zhen-hua;WU Teng-fei(Department of Cardiology,The Third Affliated Clinical Hospital of Changchun University of Traditional Chinese Medicine,Changchun 130117;Department of Cardiology,Jilin Provincial People's Hospital,Changchun 130021;Experimental Animal Department,China Medical University,Shenyang 110022,China)
出处
《解剖科学进展》
CAS
2024年第2期193-196,共4页
Progress of Anatomical Sciences
