抗敏抗炎活性物经皮共输送纳米载体安全性及功效研究

Safety and Efficacy of Transdermal Co-Delivery Nanocarriers for Anti-Sensitive and Anti-Inflammatory Ingredients

目的对包载了依克多因/甘草次酸/透明质酸钠/乙酰基二肽-1鲸蜡酯的经皮共输送纳米载体(EGHA-NCs)的安全性及抗敏抗炎功效进行研究。方法通过细胞活性测试、鸡胚尿囊膜试验、皮肤斑贴试验,研究EGHA-NCs的安全性;通过建立细胞炎症模型和细胞敏感模型,检测抗敏抗炎相关因子的分泌,研究EGHA-NCs的抗敏抗炎功效。结果EGHA-NCs安全性较好,无皮肤刺激性,能显著抑制细胞分泌TRPVl、PGE2、IL-1β、IL-8和IL-6水平,且效果明显优于同剂量游离成分溶液。结论EGHA-NCs在抗敏抗炎护肤品领域有良好的应用前景。

OBJECTIVE The safety and efficacy of transdermal co-delivery nanocarriers(EGHA-NCs)encapsulated with ectoin/glycyrrhetinic acid/sodium hyaluronate/acetyl dipeptide-1 cetyl ester foranti-allergic and anti-inflammatory were investigated.METHODS The safety of EGHA-NCs was investigated by cell activity test,chicken embryo allantoic membrane test,and skin patch test;the anti-sensitivity and anti-inflammatory efficacy of ECHA-NCs was investigated by establishing cell inflammation model and cell sensitisation model,and detecting the secretion of anti-sensitivity and anti-inflammatory related factors.RESULTS EGHA-NCs had a better safety profile without skin irritation.It could significantly inhibit the cellular secretion of TRPVI,PGE,IL-1β,IL-8,and IL-6 levels,and the effect was better than that of free ingredient solution in same dosage.CONCLUSION EGHA-NCs have good application prospects in the field of anti-sensitive and anti-inflammatory skin care products.