提高丝状真菌精准基因编辑效率的策略

Strategies for improving the efficiency of precise gene editing in filamentous fungi

基因编辑技术的应用使对丝状真菌进行高效、精准的基因改造成为可能。然而,在实际实验操作过程中,仍然存在很多影响因素,导致同源重组介导的精准基因编辑的效率很低。本文对目前提高丝状真菌精准编辑效率的方法进行了总结,使基因编辑方法更好地应用于丝状真菌的菌种改造。

The application of gene editing enables the efficient and accurate genome modification of filamentous fungi.However,the actual operation is influenced by a variety of factors,which result in low efficiency of precise gene editing mediated by homologous recombination.In this review,we summarize the current methods to improve the precision editing efficiency of filamentous fungi,so as to improve the application of gene editing in strain modification.