摘要
重组酶聚合酶扩增(recombinase polymerase amplification,RPA)技术是一种利用重组酶(T4 UvsX)、单链DNA结合蛋白(T4 Gp32)和链置换DNA聚合酶(Bsu)等蛋白在恒温条件下扩增核酸的技术。由于其可以恒温、高效地扩增靶核酸片段,近几年在细菌、病毒、寄生虫、支原体、衣原体检测方面发挥着独特的作用,逐渐成为床旁检测(point-of-care testing,POCT)的重要技术。本文以RPA技术的原理为起点,对其临床应用、不足之处及改进方法进行阐述,以期为临床疾病的快速检测和诊断方面提供新思路。
Recombinase polymerase amplification(RPA)is a technology that utilizes proteins such as recombinase(T4 UvsX),single-stranded DNA binding protein(T4 Gp32),and strand replacement DNA polymerase(Bsu)to amplify target nucleic acids at constant temperatures.In recent years,RPA plays a unique role in the detection of bacteria,viruses,parasites,mycoplasma,and chlamydia for it can amplify the target nucleic acid fragment with constant temperature and high efficiency,it gradually becomes a key technology for point-of-care testing(POCT).This review takes the principle of RPA technology as a starting point,it describes the clinical application,shortcomings,and improvement of RPA,and RPA is expected to provide a new idea for the rapid detection and diagnosis of clinical diseases.
作者
沈方园
葛萧
张晓宇
张郁勃
周慧姿
李恒
SHEN Fangyuan;GE Xiao;ZHANG Xiaoyu;ZHANG Yubo;ZHOU Huizi;LI Heng(School of Medical Laboratory,Weifang Medical University,Weifang 261053,Shandong,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2024年第5期1495-1511,共17页
Microbiology China
基金
国家自然科学基金(81902170)。
关键词
重组酶聚合酶扩增技术
等温扩增技术
核酸检测
recombinase polymerase amplification technique
isothermal amplification technique
nucleic acid testing