基于高通量测序分析中华辣椒果实发育期相关的miRNA

Analysis of miRNAs Related to the Developmental Stage of Capsicum chinense Fruit Based on High-throughput Sequencing

辣椒的成熟过程是一个受基因调控表达以及多种通路协作完成的复杂的过程,其中microRNA在调控基因表达方面具有重要的作用。本研究为了进一步探索辣椒果实中miRNA及其靶基因在果实成熟过程中的功能,构建了中华辣椒(Capsicum chinense)绿熟期、转色期、红熟期不同成熟时期果实的小RNA文库,并利用生物信息学分析探讨其调控过程中的信号通路。结果表明,三个时期分别获得22138507、17692000和14480729条miRNA序列,共鉴定出149条保守的miRNA。其中,绿熟期到转色期下调表达和上调表达的miRNA分别有3和7条,绿熟期到红熟期下调表达和上调表达的miRNA分别有26和22条,说明microRNA参与了果实成熟的过程。靶基因预测及功能富集分析显示它们可能参与了乙烯、生长素、细胞分裂素、脱落酸、茉莉酸介导的信号途径。本研究结果为今后辣椒成熟中的激素调节变化的研究提供了可能的理论依据。

The maturation process of pepper is a complex process that is regulated by gene expression and coordinated by multiple pathways. Among them, microRNA plays an important role in regulating gene expression. In this study, in order to further explore the functions of miRNA and its target genes in capsicum fruits during fruit ripening, a small RNA library of Chinese pepper(Capsicum chinense) fruits of different maturity periods in green,color, and red ripe periods was constructed, and Use bioinformatics analysis to explore the signal pathways in its regulation process. The results showed that 22 138 507, 17 692 000 and 14 480 729 miRNA sequences were obtained in the three periods, and a total of 149 conservative miRNAs were identified. Among them, there were 3and 7 miRNAs that were down-regulated and up-regulated from the green ripening stage to the color transition stage, and 26 and 22 miRNAs were down-regulated and up-regulated from the green ripening stage to the red ripening stage, indicating that microRNAs are involved in fruit ripening process. Target gene prediction and functional enrichment analysis show that they may be involved in signal pathways mediated by ethylene, auxin,cytokinin, abscisic acid, and jasmonic acid. The results of this study provide a possible theoretical basis for future research on the hormonal regulation changes in pepper ripening.