油橄榄PLATZ基因家族的鉴定及表达模式

Identification and Expression Pattern of PLATZ Gene Family in Olea europaea

PLATZ是植物中发现的一类锌指类转录因子,对于调控植物的基因表达具有重要作用。为明确油橄榄中PLATZ基因家族成员的特征,本研究对油橄榄PLATZ基因家族进行了鉴定与分析。结果表明:在油橄榄中共鉴定到29个PLATZ基因家族成员(OePLATZs);不同OePLATZs基因之间结构较为保守;染色体定位表明基因在染色体中分布比较分散,亚细胞定位结果显示其中28个OePLATZs蛋白质定位在细胞核中,而OePLATZ3蛋白质定位在线粒体中;结构预测发现二级结构中具有较大比例的无规则卷曲,三级结构中29个OePLATZs蛋白质在基序Ⅰ处多有α-螺旋的存在,在基序Ⅱ处多为是无规则卷曲;根据基因差异表达结果可将OePLATZs基因在不同组织中的表达模式分为8类,并根据干旱胁迫条件下树枝组织的转录组数据,鉴定了发生上调和下调的OePLATZs基因;系统发育分析发现油橄榄和拟南芥的PLATZ基因家族成员之间有较近的亲缘关系。本研究初步鉴定并分析了油橄榄的PLATZ基因家族成员的特征与功能,为进一步对油橄榄PLATZ家族转录因子的生物学研究提供了一定基础。

PLATZ is a zinc finger transcription factor in plants. It plays an important role in regulating gene expression. In order to clarify the characteristics of PLATZ gene family in Olea europaea, which identified and analyzed in this study. The results showed that 29 members of PLATZ gene family were identified in Olea europaea(OePLATZs). The gene structure of Oe PLATZs was relatively conservative. Chromosome localization indicated that genes were dispersed in chromosomes. Subcellular localization showed that 28 OePLATZs proteins were located in the nucleus, and OePLATZ3 protein was located in mitochondria. Structural prediction showed that there was a large proportion of random coil in the secondary protein structure, and 29 OePLATZs proteins had α-helix at motif Ⅰ and random coil at motif Ⅱ in the tertiary protein structure. According to the results of gene differential expression, the expression patterns of OePLATZs gene in different tissues were divided into 8 categories, and the up-regulated and down-regulated Oe PLATZs genes were identified based on the transcriptome data of branches under drought stress. Phylogenetic analysis showed that there was a close relationship between PLATZ gene family in Olea europaea and Arabidopsis thaliana. This study preliminarily identified and analyzed the functions of Olea europaea PLATZ gene family, which provide a basis for further biological research of Olea europaea PLATZ family transcription factors.