大发表的化学成分研究

Chemical constituents of Campylotropis trigonoclada

目的研究大发表Campylotropis trigonoclada干燥全株的化学成分。方法利用HPD-100大孔吸附树脂、硅胶、反相RP-18、凝胶Sephadex LH-20、HPLC等色谱方法进行分离纯化,并运用MS、NMR等波谱方法鉴定化合物的结构。结果从大发表中分离得到24个化合物,分别鉴定为(+)-儿茶酸(1)、芦丁(2)、槲皮素-3-O-α-L-阿拉伯吡喃糖苷(3)、广寄生苷(4)、槲皮素-3-O-葡萄糖苷(5)、槲皮素-3-O-β-D-吡喃木糖苷(6)、木犀草素-7-O-β-龙胆二糖苷(7)、荭草素(8)、异牧荆素-2″-O-β-D-吡喃葡萄糖苷(9)、voilanthin(10)、染料木素(11)、bolusanthinⅢ(12)、二羟四氢黄酮-7-O-葡萄糖苷(13)、白皮杉醇4’-O-β-D-吡喃葡萄糖苷(14)、(Z)-虎杖苷(15)、二氢菜豆酸(16)、millettiaspecoside B(17)、七叶内酯(18)、交链孢酚(19)、邻苯二甲酸二丁酯(20)、邻苯二甲酸二辛酯(21)、2,4-二羟基-3,6-二甲基苯甲酸甲酯(22)、3,4-二羟基苯甲醛(23)和没食子酸乙酯(24)。其中黄酮类化合物13个、二苯乙烯苷类化合物2个、倍半萜类化合物1个、苯酚苷类化合物1个、香豆素类化合物1个和其他芳香化合物6个。结论除化合物11外,其余23个化合物均为首次从该属植物中分离得到。

Objective To investigate the chemical constituents of Campylotropis trigonoclada.Methods The chemical constituents were isolated by the combination of HPD-100,silica gel,RP-18,Sephadex LH-20 and high performance liquid chromatography.Their structures were elucidated on the basis of spectroscopic analysis(MS,NMR,etc.).Results Twenty-four compounds were isolated from the extract of C.trigonoclada and identified as(+)-catechin(1),rutin(2),quercetin-3-Oα-L-arabinopyranoside(3),avicularin(4),quercetin-3-O-glucoside(5),quercetin-3-O-β-D-xylpyranoside(6),luteolin-7-Oβ-gentiobioside(7),orientin(8),isovitexin-2″-O-β-D-glucopyranoside(9),voilanthin(10),genistein(11),bolusanthin III(12),orobol-7-O-glucoside(13),piceatannol 4'-O-β-D-glucopyranoside(14),(Z)-polydatin(15),dihydrophaseic acid(16),millettiaspecoside B(17),esculetin(18),alternariol(19),dibutylphthalate(20),di(2-ethylhexyl)phthalate(21),methyl 2,4-dihydrox-3,6-dimethylbenzoate(22),3,4-dihydroxybenzaldehyde(23)and ethyl gallate(24).There were 13 flavonoids,two stilbene glycosides,one sesquiterpenoid,one phenol glycoside,one coumarin and six other aromatic compounds.Conclusion Except for compound 11,the other 23 compounds were isolated from the genus Campylotropis for the first time.