鸡血藤指纹图谱的建立及抗肿瘤谱效关系研究

Establishment of fingerprints and spectrum-effect relationship of anti-tumor activity of Spatholobi Caulis

目的建立鸡血藤Spatholobus suberectus HPLC指纹图谱,研究鸡血藤指纹图谱与抗肿瘤活性之间的谱效关系。方法采用HPLC法建立鸡血藤指纹图谱;以人肝癌HepG2细胞为供试细胞,采用MTT法测定鸡血藤抗肿瘤活性,运用灰色关联度和偏最小二乘法分析鸡血藤指纹图谱与抗肿瘤作用的谱效关系。结果构建鸡血藤的HPLC图谱,标出了14个共有峰,并指认了其中6个共有峰,其中2号峰儿茶素、3号峰葛根素、9号峰大豆苷元偏最小二乘标准方程回归系数为正值,与抗肿瘤活性呈正相关,且VIP值>1.0;同时,通过活性成分验证结果显示儿茶素、葛根素、大豆苷元均具可抑制HepG2细胞生长,且存在浓度相关性,可认为是鸡血藤抗HepG2细胞的物质基础。结论通过鸡血藤HPLC指纹图谱与抗肿瘤活性之间的相关性分析,为挖掘鸡血藤的质量标志物以及药材质量控制提供参考。

Objective To establish HPLC fingerprints of Jixueteng(Spatholobi Caulis)and study the spectrum-effect relationship between its fingerprints and anti-tumor activity.Methods HPLC was used to establish the fingerprint of Spatholobi Caulis.With HepG2 cells as the test cells,the anti-tumor activity of Spatholobi Caulis was determined by MTT method.The spectrum-effect relationship between Spatholobi Caulis fingerprint and anti-tumor activity was analyzed by grey correlation degree and partial least square method.Results HPLC chromatogram of Spatholobi Caulis was established,a total of 14 common peaks were identified,and six of them were identified.The partial least square standard equation regression coefficients of peak 2 catechin,peak 3 puerarin and peak 9 daidzein were positive,positively correlated with anti-tumor activity,and VIP value was>1.0.The verification results of active ingredients showed that catechin,puerarin and daidzein could inhibit HepG2 cell growth in a concentration dependent manner,which could be considered as the material basis of anti-HepG2 cells of Spatholobi Caulsi.Conclusion Through the correlation analysis between the anti-tumor activity and the HPLC fingerprint of Spatholobi Caulis,this study can serve as a reference for excavating the quality markers of Spatholobi Caulis and the quality control of medicinal materials.