河南省商丘市猫泛白细胞减少症的病原PCR检测及VP2基因序列分析

PCR Detection and Sequence Analysis of VP2 Gene of Leukopenia Panacea in Cats from Shangqiu,He'nan Province

猫泛白细胞减少症(FP)是由猫细小病毒(FPV)引起的一种急性高度接触性传染病,为了解商丘市FPV的遗传变异情况,从该市2家宠物医院采集18份疑似FPV样品,通过DNA提取及PCR特异性检测提取出14份阳性样品,对该14份阳性样品进行细小病毒结构蛋白2(VP2)基因进行PCR扩增,测序和遗传进化分析。结果表明,该14份阳性样品中有12个毒株与中国FPV(G1)MF541135毒株关系亲密,其同源性均与参考株FPV(G1)MF541135的同源性最高,该12份阳性样品均属于G1型;另外2个毒株FPV-SQ-09、FPV-SQ-12与参考株CPV(MW182708)关系亲密,且同源性较高。遗传进化树显示,FPV G1型均来源于中国,研究的大部分阳性样品VP2基因有亚洲起源的特征。该文为研究FPV在商丘地区的传播和宠物疫病防控提供理论依据。

Feline leukopenia(FP)is an acute,highly contagious disease caused by feline parvovirus(FPV).To understand the genetic variation of FPV in Shangqiu,18 suspected FPV samples were collected from two pet hospitals in the city,and 14 positive samples were obtained by DNA extraction and PCR specific detection,the structural protein 2(VP2)gene of parvovirus was amplified by PCR,sequenced and analyzed by phylogenetic analysis.The results showed that 12 of the 14 positive samples were closely related to the Chinese FPV(G1)MF541135 strain and had the highest homology with the reference strain FPV(G1)MF541135.All the 12 positive samples belonged to G1type.The other two strains,FPV-SQ-09 and FPV-SQ-12,were closely related to the reference strain CPV(MW182708).The genetic tree showed that all of the FPV G1 types originated from China,and most of the VP2 genes in the study had characteristics of Asian origin.This paper provides a theoretical basis for the study of the transmission of FPV in Shangqiu and the prevention and control of pet diseases.