简便枕大池二次注血法建立脑血管痉挛模型的效果分析

Cerebral vasospasm model established by brief double blood injection in cisternal cisteria:an efficacy analysis

目的探讨简便枕大池二次注血法建立脑血管痉挛模型的效果。方法25只SD大鼠按随机数字表法分为假手术组、蛛网膜下腔出血(SAH)后1 d组、SAH后3 d组、SAH后5 d组、SAH后7 d组,每组5只。后4组大鼠采用简便枕大池二次注血法,即穿刺尾动脉获取自体血0.2 mL后直接穿刺寰枕膜注射自体血,48 h后重复上述操作建立脑血管痉挛模型。采用改良神经功能缺损程度评分评估各组大鼠神经功能缺损程度,采用HE染色检测各组大鼠基底动脉(BA)的直径和横截面积。比较各组大鼠造模前体质量、2次注血前体质量差、神经功能缺损程度评分、BA直径和横截面积的差异。结果(1)5组大鼠造模前体质量的差异无统计学意义(P>0.05)。与假手术组比较,SAH后1 d组、SAH后3 d组、SAH后5 d组、SAH后7 d组大鼠2次注血时体质量差较大,差异有统计学意义(P<0.05)。(2)与假手术组比较,SAH后1 d组、SAH后3 d组、SAH后5 d组、SAH后7 d组大鼠神经功能缺损程度评分较高,差异均有统计学意义(P<0.05)。(3)与假手术组比较,SAH后3 d组、SAH后7 d组大鼠BA直径较短,且SAH后7 d组大鼠BA直径短于SAH后1 d组、SAH后5 d组,差异均有统计学意义(P<0.05)。与假手术组比较,SAH后1 d组、SAH后3 d组、SAH后5 d组、SAH后7 d组大鼠BA横截面积均较小,且SAH后7 d组大鼠BA横截面积小于SAH后1 d组、SAH后3 d组、SAH后5 d组,差异均有统计学意义(P<0.05)。结论采用简便枕大池二次注血法建立SAH后脑血管痉挛模型,具有操作简化、造模时间短、微创等优点,且以注血后7 d最合适。

Objective To explore the efficacy of cerebral vasospasm model established by brief double blood injection in cisternal cisteria.Methods Twenty-five SD rats were randomly divided into sham-operated group,group of 1 d after subarachnoid hemorrhage(SAH),group of 3 d after SAH,group of 5 d after SAH,and group of 7 d after SAH(n=5).Autologous blood(0.2 mL,obtained by caudal artery puncture)was directly injected into the atlanto-occipital membrane and repeated 48 h after that to establish cerebral vasospasm model.Neurological impairment was evaluated by modified Neurological Severity Score(mNSS).Diameter and cross-sectional area of the basilar artery(BA)were detected by HE staining.Differences of body mass before modeling,body mass between the 2 blood injections,mNSS scores,and diameter and cross-sectional area of BA were compared among groups.Results(1)Body mass before modeling was not significantly different among the 5 groups(P>0.05);differences of body mass between the 2 blood injections in the group of 1 d after SAH,group of 3 d after SAH,group of 5 d after SAH,and group of 7 d after SAH were significantly greater than those in the sham-operated group(P<0.05).(2)The mNSS scores in the group of 1 d after SAH,group of 3 d after SAH,group of 5 d after SAH,and group of 7 d after SAH were significantly higher than those in the sham-operated group(P<0.05).(3)BA diameter in the group of 3 d after SAH and group of 7 d after SAH was significantly shorter than that in the sham-operated group,and that in the group of 7 d after SAH was significantly shorter than that in the group of 1 d after SAH and group of 5 d after SAH(P<0.05).BA cross-sectional area in the group of 1 d after SAH,group of 3 d after SAH,group of 5 d after SAH and group of 7 d after SAH was significantly smaller than that in the sham-operated group,and that in the group of 7 d after SAH was significantly smaller than that in the group of 1 d after SAH,group of 3 d after SAH and group of 5 d after SAH(P<0.05).Conclusion Compared with other traditional models,cerebral vasospasm model established by brief double blood injection in cisternal cisteria has advantages of simplified operation,short modeling time,and minimal invasion;model of 7 d after autologous blood injection enjoys optimal.