HBsAg阳性孕产妇HBV宫内感染危险因素及其与IL-28B基因多态性的关联

Risk factors for intrauterine HBV infection in HBsAg-positive pregnant women and association with IL-28B gene polymorphisms

目的探讨乙型肝炎病毒(HBV)携带孕产妇HBV宫内感染危险因素及其与白细胞介素-28B(IL-28B)基因多态性的关联。方法选择济南市第二妇幼保健院2019年1月-2022年2月收治的620例HBsAg阳性孕产妇为研究对象,其中50例发生HBV宫内感染患者为感染组,570例未发生HBV宫内感染患者为未感染组,统计两组IL-28B基因rs8099917位点多态性,分析HBV宫内感染危险因素。结果感染组GG基因型及G等位基因频率均高于未感染组(P<0.05);HBsAg阳性孕产妇HBV宫内感染的危险因素包括IL-28B基因GG基因型、G等位基因、HBV DNA>10^(6)copies/ml、肝脏B超改变及前S1蛋白阳性(P<0.05)。结论HBsAg阳性孕产妇HBV宫内感染与IL-28B基因多态性、HBV DNA水平、肝脏B超改变、前S1蛋白阳性有关。

OBJECTIVE To explore the risk factors for intrauterine hepatitis B virus(HBV)infection in the pregnant women carried with HBV and analyze the association with gene polymorphisms of interleukin-28B(IL-28B).METHODS A total of 620 HBsAg-positive pregnant women who were treated in Jinan Second Maternal and Child Health Hospital from Jan 2019 to Feb 2022 were recruited as the research subjects,50 of whom had intrauterine HBV infection and were assigned as the infection group,and 570 who did not have intrauterine infection and were assigned as the no infection group.The polymorphisms at rs8099917 locus of IL-28B gene were statistically analyzed,and the risk factors for the intrauterine HBV infection were observed.RESULTS The frequencies of GG genotype and G allele of the infection group were significantly higher than those of the no infection group(P<0.05).The risk factors for the intrauterine HBV infection in the HBsAg-positive pregnant women included the GG genotype and G allele of IL28 gene,HBV DNA more than 10^(6) copies/ml,change of liver B-ultrasound and positive pre S1 protein(P<0.05).CONCLUSION The intrauterine HBV infection of the HBsAg-positive pregnant women is associated with the IL-28B gene polymorphisms,HBV DNA level,change of liver B-ultrasound and positive pre S1 protein.