载中药聚乳酸多孔纳米纤维医用敷料

Medical Dressing Prepared from Polylactic Acid Porous Nanofiber Loaded Chinese Drugs

为构筑人体可吸收、抗菌消炎且轻薄柔软的可用于伤口愈合的医用敷料,以聚乳酸为原料,掺杂板蓝根(RI)、柴胡(BC)和茶多酚(GTP)等中药成分,采用氯仿与丙酮二溶剂体系,借助静电纺技术制备聚乳酸(PLA)多孔纳米纤维载中药敷料。通过扫描电镜、红外光谱仪、X射线衍射仪(XRD)和接触角测试仪等手段分析了载中药敷料的微观结构、润湿性能、保水吸液性、抗菌性以及体外降解性能等。结果表明:采用氯仿和丙酮二溶剂体系制备的载中药敷料其纤维具有多孔特征,纤维直径为747~1 550 nm。敷料中所载板蓝根、柴胡、茶多酚与聚乳酸间并未发生负面改性的化学反应。载板蓝根、柴胡、茶多酚的敷料在载药量为3%时对金黄色葡萄球菌抑菌率分别为79.2%、82.5%、75.7%,对大肠杆菌抑菌率分别为79.1%、80.2%、73.9%。同时,较纯聚乳酸敷料,载中药敷料具有较好的透气性和体外降解性,其保水吸液性均提高20%以上,适合用作伤口敷料。

In order to construct a medical dressing with the absorbability by human body,antibacterial and anti-inflammatory property,thin and soft characteristic for wound healing,polylactic acid(PLA)porous nanofibers dressings loaded with traditional Chinese medicines of radix isatidis(RI),bupleurum chinense(BC)and green tea polyphenols(GTP)were prepared by electrospinning technique,which used the mixture of chlo-roform and acetone as solvent system.By means of scanning electron microscope,infrared spectrometer,X-ray diffractometer(XRD)and contact angle tester,the microstructure,wettability,water retention and liquid absorption,antibacterial and in vitro degradation of the dressing were analyzed.The results show that the nanofibers of dressing loaded Chinese medicine prepared by chloroform and acetone two solvent system had porous characteristics,and the fiber diameter was about 747—1550 nm.There was no negative modification reaction between Chinese medicine ingredients and polylactic acid.The antibacterial rates of the dressings loaded with radix isatidis,bupleuri chinense and green tea polyphenols against staphylococcus aureus were 79.2%,82.5%and 75.7%respectively when the loading drug content was 3%,and the antibacterial rates against Escherichia coli were 79.1%,80.2%and 73.9%respectively.At the same time,compared with pure polylactic acid dressings,the dres-sings loaded drug have better air permeability and in vitro degradation performance,and their water retention and liquid absorption properties are improved by more than 20%,which is suitable for use as wound dressings.