摘要
利用液相色谱仪、制备液相色谱仪、气相色谱质谱联用仪,对歧化松香(DR)中去氢枞酸(DA)紫外光谱测定方法的干扰因素进行了详细研究。测定的市售不同省份的5个样品中有2个主要干扰物质,进一步定性,确定干扰物质1为6,8,11,13-枞四烯酸(ATA),干扰物质2为混合物,主要检出物为去氢枞醛(D)和枞酸(AA)。采用二阶导数光谱法和偏最小二乘(PLS)法对紫外光谱数据进行分析,以消除去氢枞酸紫外分光光度法的干扰,结果表明:PLS法比二阶导数光谱法误差更小,能够同时消除多种干扰因素,使测试结果更加接近真实值。
The interference factors for the determination method of dehydroabietic acid(DA)in disproportionated rosin(DR)by UV spectrophotometry were investigated in detail using liquid chromatography,preparative liquid chromatography and gas chromatography-mass spectrometry.The results showed that there are two main interference substances in five purchased samples produced from different provinces.Further identification determined that interference substance one was 6,8,11,13-abietatetraenoic acid(ATA),and the other was a mixture main containing mainly dehydroabietal(D)and abietic acid(AA).The spectral data were analyzed using the second-order derivative spectrophotometric and partial least squares(PLS)method to eliminate the interference for the UV spectrophotometry determination method of dehydroabietic acid.The results indicated that PLS method had smaller error value than the second-order derivative spectrophotometric method and could eliminate multiple interference factors simultaneously,which made the test results closer to the actual values.
作者
王宏晓
沈娟章
马艳
谭卫红
WANG Hongxiao;SHEN Juanzhang;MA Yan;TAN Weihong(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China)
出处
《林产化学与工业》
CAS
CSCD
北大核心
2024年第3期30-36,共7页
Chemistry and Industry of Forest Products
基金
国家自然科学基金面上项目(32071708)。
关键词
歧化松香
紫外可见分光光度法
干扰因素
偏最小二乘法
disproportionated rosin
UV-visible spectrophotometry
interference factor
partial least squares method
