自噬流的诱导与检测综合性实验的设计与实践

Design and Practice of the Comprehensive Experiment of Induction and Detection of Autophagic Flux

将国际前沿理论和技术拆解和提炼及时补充到实验教学中,是提高实验教学水平和人才培养质量的重要途径之一。该研究以科研成果为基础设计了“自噬流的诱导和检测”综合性实验,包含细胞传代培养、细胞转染、自噬诱导与观察3个部分,形成系统性、综合性和探究性的模块化实验教学内容,通过细胞培养、转染技术在细胞中表达双荧光标记自噬体标志蛋白,经饥饿诱导细胞,在荧光显微镜下观察自噬体和自噬流变化。在本科生实验教学中的实践结果表明,该实验有助于培养学生科研思维和创新意识、提升学生分析问题和解决问题的能力。

One of the important ways to improve the experimental teaching level and the talent training quality is dismantling,refining and adding the international frontier theories and technologies to the experimental teaching in time.The comprehensive experiment of induction and detection of autophagic flux is designed,which includes cell passage culture,cell transfection and induction and observation of autophagy.A systematic,comprehensive and exploratory modular experimental teaching content is formed.Through double fluorescent labeled autophagosome marker protein expressed in cells and starvation inducing,autophagosomes and autophagic flux are observed by the fluorescence microscope.The practice results in undergraduate experimental teaching indicate that it is helpful to cultivate students’scientific research thinking and the innovative spirit,and improve their ability to analyze and solve problems.