长链非编码RNA OTUD6B-AS1对肺腺癌A549细胞增殖、侵袭和迁移能力的影响

Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells

目的探讨lncRNA OTUD6B-AS1对肺腺癌A549细胞增殖、迁移和侵袭能力的影响。方法体外培养肺腺癌A549细胞系,瞬时转染OTUD6B-AS1和空载质粒构建过表达和对照细胞模型,分为OTUD6B-AS1过表达组和空载质粒组(NC组)。通过qRT-PCR实验验证OTUD6B-AS1的转染效率;采用CCK-8实验检测OTUD6B-AS1对肺腺癌细胞增殖活性的影响,采用Transwell法检测OTUD6B-AS1对肺腺癌细胞迁移和侵袭能力的影响。结果qRT-PCR检测显示,与NC组相比,过表达组的OTUD6B-AS1表达量显著增高(P<0.05);CCK-8细胞活性检测结果显示,OTUD6B-AS1过表达组的肺腺癌A549细胞增殖活性较NC组显著降低(P<0.05);Transwell侵袭实验结果表明,OTUD6B-AS1过表达组细胞迁移和侵袭能力均显著低于NC组(P<0.05)。结论在肺腺癌细胞A549中lncRNA OTUD6B-AS1的过表达可以抑制A549细胞增殖、迁移和侵袭的能力。

Objective The aim of this study was to investigate the effects of LncRNA OTUD6B-AS1 on the proliferation,migration and invasion of lung adenocarcinoma A549 cells.Methods Lung adenocarcinoma A549 cell line was cultured in vitro,and transient transfection of OTUD6B-AS1 and empty plasmid group were used as the control group.Overexpression and control cell models were constructed,and divided into OTUD6B-AS1 overexpression group and empty plasmid group(NC group).The cell model was divided into the empty plasmid group(NC group)and OTUD6B-AS1 overexpression group.The transfection efficiency of OTUD6B-AS1 mRNA was verified through qRT-PCR.The CCK-8 experiment was used to detect the effect of OTUD6B-AS1 on the proliferation activity of lung adenocarcinoma cells,and the Transwell assay was used to detect the effect of OTUD6B-AS1 on the migration and invasion ability of lung adenocarcinoma cells.Results Compared to the NC group,the overexpression OTUD6B-AS1 group had a significant increase in the expression of OTUD6B-AS1(P<0.05).The CCK-8 assay results showed that the proliferation activity of A549 cells in the OTUD6B-AS1 overexpression group was significantly reduced compared to the NC group(P<0.05).The results of the Transwell assay showed that the OTUD6B-AS1 overexpression group had significantly lower cell migration and invasion abilities than the NC group(P<0.05).Conclusion Overexpression lncRNA OTUD6B-AS1 in lung adenocarcinoma A549 cells can significantly inhibit the proliferation,migration,and invasion ability of A549 cells.