一起仔兔皮下脓肿的诊断

Diagnoses of the Subcutaneous Abscess in Rabbit

2023年9月福建省某兔场发生一起病因不明的仔兔皮下脓肿,脓肿发生在仔兔的颈背部或背部,脓肿约鸽子蛋至鸡蛋大小。为确定此起仔兔皮下脓肿的病因,采集了病变部位的脓汁,从脓汁中分离到1株菌落为半透明灰白色的革兰阴性杆菌PX01。经与NCHI数据库中已知序列的BLSAT比对分析,分离菌PX01的16S rRNA基因序列与多杀性巴氏杆菌的16S rRNA基因序列同源性最高,高达99.8%~100%。根据分离菌PX01的菌落形态、革兰染色结果和16S rRNA基因序列,确定该分离菌为多杀性巴氏杆菌。此外,分离菌PX01对卡那霉素等7种药物不敏感或中度敏感,建议临床上对该菌的防控应科学用药。

In September 2023,a subcutaneous abscess with unknown cause occurred in a rabbit farm in Fujian Province.The abscess occurred under the neck or back of the rabbit.The diameter of the abscess was about the size of the pigeon egg to the egg.To determine the causative agent of the subcutaneous abscess in the rabbits,the pus from the lesion site of the rabbits was collected,and a Gram-negative bacterium with translucent gray-white colony named PX01 was recovered from the pus.By using BLAST analyses,the 16S rRNA gene sequence of PX01 showed the highest identity(ranged from 99.8~100%)with that of Pasteurella multocida.Based on the features of colonial morphology,Gram-staining and the sequence of 16S rRNA,the PX01 was identified as the Pasteurella multocida.Furthermore,the PX01 was resistant to or intermediately susceptible to 7 out of 12 tested drugs,sug⁃gesting that the prevention and control of the PX01 should base on the results of drug sensitivity tests.