防城金花茶叶片的生药学及UPLC指纹图谱

The pharmacognosy and UPLC fingerprint of the leaves of Camellia nitidissima var.phaeopubisperma

采用性状鉴别和显微鉴别方法,观察防城金花茶(Camellia nitidissima var.phaeopubisperma)的原植物形态、组织结构、粉末特征;采用超高效液相色谱(UPLC)并运用中药色谱指纹图谱相似度评价系统建立防城金花茶液相指纹图谱,运用SIMCA-P14.1软件对其化学成分进行主成分分析和正交偏最小二乘法-判别分析。性状鉴别结果显示,防城金花茶叶展开后呈椭圆形、长椭圆形、矩圆形或卵圆形,薄革质、两面均无毛,叶脉网状,边缘有细锯齿或锯齿。叶肉组织散有大型分枝状不规则石细胞和草酸钙簇晶;导管有环纹导管及螺纹导管,气孔不定式。超高液相指纹图谱显示,供试防城金花茶样本化学成分谱存在差异;基于化学成分差异,采用主成分分析和正交偏最小二乘法-判别分析可将防城金花茶样本分成2大类,维生素B6、去氧肾上腺素、芦丁、咖啡碱、金丝桃苷是区分2种指纹类型的主要标志性成分。

The plant morphology,tissue structure and powder characteristics of Camellia nitidissima var.phaeopubisperma were ob-served by means of character identification and microscopic identification.The liquid fingerprint of Camellia nitidissima var.phaeopubi-sperma was established by ultra high-performance liquid chromatography(UPLC)and similarity evaluation system of traditional Chi-nese medicine chromatographic fingerprint.Principal component analysis and orthogonal partial least squares discriminant analysis were performed on the chemical composition by SIMCA-P14.1 software.The results of character identification showed that Camellia nitidissima var.phaeopubisperma was elliptical,oblong,rectangular or oval,thin leathery,hairless on both sides,reticulated leaf veins and serrulate or serrate on the margins.In the mesophyll tissue were present large branching irregular stone cells and clusters of calcium oxalate crystals;the vessels were annular and spiral,and the stomata were anomocytic type.The HPLC fingerprints indicated differences in the chemical composition of the tested Camellia nitidissima var.phaeopubisperma samples.Based on these differences,the samples could be divided into two categories using principal component analysis and orthogonal partial least squares-discriminant analysis,with vitamin B6,phenylephrine,rutin,caffeine,and hyperoside being the key signature ingredients that distinguished the two types of fingerprints.