中小叶种茶酯型儿茶素含量测定及亚细胞定位

Content determination and subcellular localization of ester catechins of small and medium-leaf teas

为了明确酯型儿茶素在中小叶种茶叶中的亚细胞空间定位,以十里香、桂绿1号、浙农113、福鼎大白茶、浙农139共5种中小叶种茶样为试验材料,采用高效液相色谱法对5种茶样中的酯型儿茶素含量进行检测,采用中性红染色法对茶样细胞中液泡进行定位,通过免疫组织化学法对茶样中酯型儿茶素进行亚细胞定位。结果表明,5种中小叶种茶均含有不同含量的酯型儿茶素,其中含量最高的为十里香茶;在中性红染色情况下,十里香茶样中液泡呈圆形或椭圆形;免疫组织化学法染色情况下,5种中小叶种茶酯型儿茶素在亚细胞水平上均定位于液泡中。

In order to clarify the subcellular spatial localisation of ester catechins in small and medium-leaf teas,five small-and me-dium-leaf tea samples of Shilixiang,Guilv No.1,Zhenong 113,Fuding Dabai Tea and Zhenong 139 were taken as the test materials,and the content of ester catechins in the five tea samples was detected using high-performance liquid chromatography(HPLC),and the vesicles in the cells of the samples were localised using neutral red staining.Subcellular localisation of ester catechins in tea sam-ples was performed by immunohistochemistry.The results showed that all five types of small and medium-leaf teas contained different levels of ester catechins,with the highest content in Shilixiang tea;under neutral red staining,the vesicles in Shilixiang tea samples were round or oval;under immunohistochemistry staining,the ester catechins of the five types of small and medium-leaf teas were lo-cated in the vesicles at the subcellular level.