慢性牙周炎患者血清和龈沟液LncRNA MALAT1和miR-769-5p水平表达及其临床意义

Expression and Clinical Significance of LncRNA MALAT1 and miR-769-5p in Serum and Gingival Crevicular Fluid of Patients with Chronic Periodontitis

目的:探讨慢性牙周炎患者血清和龈沟液长链非编码核糖核酸肺腺癌转移相关转录子1 (LncRNA MALAT1)和微小RNA-769-5p(miR-769-5p)水平表达及其临床意义。方法:选择2020年6月~2023年7月我院收治的慢性牙周炎患者117例纳入观察组,根据慢性牙周炎患者的病情严重程度分为轻度组39例、中度组42例和重度组36例,另选取117例健康志愿者纳入对照组。对比对照组、观察组血清和龈沟液LncRNA MALAT1和miR-769-5p表达水平。对比不同病情严重程度血清和龈沟液LncRNA MALAT1和miR-769-5p表达水平、血清和龈沟液炎症指标[肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、干扰素-γ(IFN-γ)]水平、牙周指标[牙周袋深度(PD)、附着丧失(AL)、龈沟出血指数(SBI)、菌斑指数(PLI)]。采用Pearson相关性分析法分析慢性牙周炎患者炎症指标、牙周指标和血清和龈沟液miR-769-5p、LncRNA MALAT1表达水平的相关性。采用受试者工作特征(ROC)曲线分析血清和龈沟液LncRNA MALAT1、miR-769-5p对慢性牙周炎的诊断价值。结果:观察组血清和龈沟液LncRNA MALAT1表达水平高于对照组(P<0.05),血清和龈沟液miR-769-5p表达水平低于对照组(P<0.05)。轻度组的血清和龈沟液LncRNA MALAT1表达水平及IL-6、TNF-α、IFN-γ、PD、AL、SBI、PLI低于中度组(P<0.05),中度组低于重度组(P<0.05);血清和龈沟液miR-769-5p表达水平轻度组高于中度组(P<0.05),中度组高于重度组(P<0.05)。慢性牙周炎患者血清和龈沟液LncRNA MALAT1表达水平与IL-6、TNF-α、IFN-γ、PD、AL、SBI、PLI呈正相关(P<0.05),血清和龈沟液miR-769-5p表达水平与IL-6、TNF-α、IFN-γ、PD、AL、SBI、PLI呈负相关(P<0.05),血清和龈沟液LncRNA MALAT1表达水平与miR-769-5p表达水平呈负相关(P<0.05)。血清LncRNA MALAT1、miR-769-5p及联合检测诊断慢性牙周炎的曲线下面积(AUC)分别为0.800、0.743和0.877;龈沟液LncRNA MALAT1、miR-769-5p及联合检测诊断慢性牙周炎的AUC分别为0.786、0.849和0.891。结论:慢性牙周炎患者血清和龈沟液LncRNA MALAT1表达水平升高,miR-769-5p表达水平降低,可加重炎症反应和牙周炎症状,血清和龈沟液LncRNA MALAT1、miR-769-5p联合检测对慢性牙周炎具有较高的诊断价值。

Objective:To investigate the expression and clinical significance of long non-coding RNA lung adenocarcinoma metastasis-associated lung adenocarcinoma transcript 1(LncRNA MALAT1)and microRNA-769-5p(miR-769-5p)in serum and gingival crevicular fluid of patients with chronic periodontitis.Methods:117 patients with chronic periodontitis who were admitted to our hospital from June 2020 to July 2023 were selected as observation group,patients were divided into 39 cases in mild group,42 cases in moderate group and 36 cases in severe group according to the severity of chronic periodontitis,another 117 healthy volunteers were selected as control group.The expression levels of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid were compared between control group and observation group.The expression levels of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid,the levels of inflammatory indexes[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interferon-γ(IFN-γ)]in serum and gingival crevicular fluid,periodontal indexes[periodontal pocket depth(PD),attachment loss(AL),gingival crevicular bleeding index(SBI),plaque index(PLI)]were compared among different disease severity.The correlation between inflammatory indicators,periodontal indicators and the expression levels of miR-769-5p and LncRNA MALAT1 in serum and gingival crevicular fluid in patients with chronic periodontitis were analyzed by Pearson correlation analysis.The diagnostic value of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid for chronic periodontitis were analyzed by receiver operating characteristic(ROC)curve.Results:The expression levels of LncRNA MALAT1 in serum and gingival crevicular fluid in observation group were higher than those in control group(P<0.05),and the expression levels of miR-769-5p in serum and gingival crevicular fluid were lower than those in control group(P<0.05).The expression levels of LncRNA MALAT1 in serum and gingival crevicular fluid and IL-6,TNF-α,IFN-γ,PD,AL,SBI and PLI in mild group were lower than those in moderate group(P<0.05),and moderate group were lower than those of severe group(P<0.05).The expression levels of miR-769-5p in serum and gingival crevicular fluid were higher in mild group than in moderate group(P<0.05),and moderate group were higher than those of severe group(P<0.05).The expression level of LncRNA MALAT1 in serum and gingival crevicular fluid of patients with chronic periodontitis was positively correlated with IL-6,TNF-α,IFN-γ,PD,AL,SBI and PLI(P<0.05).The expression level of miR-769-5p in serum and gingival crevicular fluid was negatively correlated with IL-6,TNF-α,IFN-γ,PD,AL,SBI and PLI(P<0.05).The expression level of LncRNA MALAT1 in serum and gingival crevicular fluid was negatively correlated with the expression level of miR-769-5p(P<0.05).The area under the curve(AUC)of serum LncRNA MALAT1,miR-769-5p and combined detection in the diagnosis of chronic periodontitis were 0.800,0.743 and 0.877 respectively.The AUC of gingival crevicular fluid LncRNA MALAT1,miR-769-5p and combined detection in the diagnosis of chronic periodontitis were 0.786,0.849 and 0.891 respectively.Conclusion:The expression level of LncRNA MALAT1 in serum and gingival crevicular fluid of patients with chronic periodontitis is increase,and the expression level of miR-769-5p is decrease,which can aggravate the inflammatory reaction and periodontitis symptoms,the combine detection of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid has high diagnostic value for chronic periodontitis.