IL-35、Beclin-1在乳腺癌患者外周血中的水平及意义

Level and significance of IL-35 and Beclin-1 in peripheral blood of patients with breast cancer

目的探讨白细胞介素(IL)-35、自噬相关蛋白Beclin-1在乳腺癌患者外周血中的水平及意义。方法选取2022年5-10月在上海市嘉定区妇幼保健院诊治的原发性乳腺癌女性患者44例(乳腺癌组)、乳腺良性肿瘤女性患者40例(乳腺良性肿瘤组)及体检的健康者40例(健康对照组)作为研究对象。采用酶联免疫吸附试验检测并比较各组外周血中IL-35和Beclin-1水平,同时检测乳腺癌患者糖类抗原15-3(CA15-3),比较不同临床病理特征的乳腺癌患者IL-35、Beclin-1、CA15-3水平;分析乳腺癌患者外周血IL-35、Beclin-1、CA15-3水平之间的相关性。结果乳腺癌组和乳腺良性肿瘤组外周血IL-35水平均高于健康对照组(P<0.05),Beclin-1水平均低于健康对照组(P<0.05)。乳腺癌组外周血IL-35水平高于乳腺良性肿瘤组(P<0.05),Beclin-1水平低于乳腺良性肿瘤组(P<0.05)。不同病理分级、淋巴结转移情况的乳腺癌患者外周血IL-35、Beclin-1水平比较,差异均有统计学意义(P<0.05);不同雌激素受体(ER)检测结果的乳腺癌患者外周血IL-35水平比较,差异无统计学意义(P>0.05),而Beclin-1水平比较,差异有统计学意义(P<0.05)。乳腺癌患者外周血IL-35水平与Beclin-1水平呈负相关(r=-0.484,P<0.05);IL-35、Beclin-1水平与CA15-3水平均无相关性(均P>0.05)。结论乳腺癌患者外周血IL-35水平升高、Beclin-1水平降低。不同病理分级、淋巴结转移情况的乳腺癌患者外周血IL-35、Beclin-1水平有明显差异。乳腺癌患者外周血IL-35水平与Beclin-1水平呈负相关,而IL-35、Beclin-1水平与肿瘤标志物CA15-3水平均无关。

Objective To investigate the level and significance of interleukin(IL)-35 and autophagy related protein Beclin-1 in peripheral blood of the patients with breast cancer.Methods Forty-four female patients with primary breast cancer(breast cancer group),40 female patients with benign breast tumor(benign breast tumor group),and 40 healthy people undergoing physical examination(healthy control group)diagnosed and treated in this hospital from May to October 2022 were selected as the study subjects.The enzyme linked immunosorbent assay was used to detect and compare the levels of peripheral blood plasma IL-35 and Beclin-1 among the groups,meanwhile the clinical detection results of carbohydrate antigen 15-3(CA15-3)in the patients with breast cancer were also detected.The levels of IL-35,Beclin-1 and CA15-3 were compared among different clinical and pathological characteristics of the patients with breast cancer;the correlation among the levels of IL-35,Beclin-1 and CA15-3 in peripheral blood of the patients with breast cancer was analyzed.Results The peripheral blood IL-35 levels in the breast cancer group and benign breast tumor group were higher than those in the healthy control group(P<0.05),and the Beclin-1 level was lower than that in the healthy control group(P<0.05).The peripheral blood IL-35 level in the breast cancer group was higher than that in the breast benign tumor group(P<0.05),and the Beclin-1 level was lower than that in the breast benign tumor group(P<0.05).The peripheral blood IL-35 and Beclin-1 levels had statistically significant difference among different pathological grades and lymph node metastasis of the patients with breast cancer(P<0.05);there was no statistically significant difference in the peripheral blood IL-35 level among different estrogen receptor(ER)detection results of the patients with breast cancer(P>0.05),but there was statistically significant difference in the Beclin-1 level(P<0.05).The peripheral blood IL-35 level in the patients with breast cancer was negatively correlated with the Beclin-1 level(r=-0.484,P<0.05);there was no correlation between the IL-35 levels with Beclin-1 and CA15-3 levels(all P>0.05).Conclusion The peripheral blood IL-35 and Beclin-1 levels in the patients with breast cancer are increased and the Beclin-1 level is decreased.There are significant differences in the levels of peripheral blood IL-35 and Beclin-1 among different pathological grades and lymph node metastasis of the patients with breast cancer.IL-35 level is negatively correlated with Beclin-1 level,but IL-35 and Beclin-1 levels have no correlation with tumor marker CA15-3 level.