瘢痕疙瘩发病机制中的脂代谢相关基因:基于生物信息学分析和验证

Lipid metabolism-related genes in the pathogenesis of keloid:Based on bioinformatics analysis and validation

目的基于生物信息学方法探讨瘢痕疙瘩发生发展的脂代谢相关基因。方法从GEO数据库获取瘢痕疙瘩相关数据集,从Genecards数据库下载脂代谢相关基因,进行交集分析筛选出共同基因;对差异基因进行GO、KEGG富集分析;使用实时荧光定量PCR(qRT-PCR)和免疫组化验证显著差异基因。结果瘢痕疙瘩中脂代谢相关差异基因共有2个共同基因ACSS3和PRKD1,其中ACSS3差异最显著。差异基因的生物过程主要富集在甘油酯生物合成过程和脂肪酸衍生物代谢过程等。KEGG主要富集在甘油酯代谢过程和花生四烯酸代谢过程等。qRT-PCR和免疫组化显示ACSS3在瘢痕疙瘩组织中高表达。结论ACSS3可能通过影响脂代谢在瘢痕疙瘩发生发展中发挥重要作用。

Objective To explore lipid metabolism-related genes that contribute to the development and progression of keloid based on bioinformatics.Methods The keloid related datasets were obtained from the GEO database,while lipid metabolism-related genes were downloaded from the Genecards database.Intersection analysis was performed to screen for common genes.GO and KEGG enrichment analyses of differentially expressed genes were performed.Significant differentially expressed genes were validated using real-time fluorescence quantitative PCR(qRT-PCR)and immunohistochemistry.Results ACSS3 and PRKD1 were the two common genes related to lipid metabolism in keloid,with ACSS3 showing the most significant difference.The biological processes of differentially expressed genes were mainly enriched in the biosynthesis of glycerides and the metabolism of fatty acid derivatives.KEGG pathways were mainly associated with the processes of glyceride metabolism and arachidonic acid metabolism.Immunohistochemistry and qPCR revealed high expression levels of ACSS3 in keloid tissue.Conclusion ACSS3 may play an important role in the occurrence and development of keloid by affecting lipid metabolism.