宫颈脱落细胞中mybl2 mRNA的表达在LSIL患者中的分流价值

Shunting value on the expression of cervical exfoliated cells mybl2 mRNA in LSIL patients

目的 探究宫颈脱落细胞中mybl2 mRNA的表达在宫颈低级别鳞状上皮内病变(LSIL)患者中的分流价值。方法 180例宫颈病变患者,根据病理结果分为宫颈炎(NC)组(40例)、LSIL组(100例)及宫颈高级别鳞状上皮内病变(HSIL)组(40例)。比较三组的宫颈脱落细胞中mybl2 mRNA及人乳头瘤病毒(HPV) E6/E7 mRNA表达量、阳性率;比较LSIL组中不同转归者的宫颈脱落细胞中mybl2m RNA及HPV E6/E7 mRNA表达量、阳性率;分析LSIL患者宫颈脱落细胞中mybl2 mRNA与HPV E6/E7 mRNA的相关性。结果 NC组的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA表达量分别为(1.23±0.16)、(1.76±0.32), LSIL组分别为(3.39±0.59)、(5.01±1.32), HSIL组分别为(6.56±0.93)、(8.10±1.69)。三组的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA表达量比较,差异有统计学意义(P<0.05);HSIL组的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA表达量显著高于NC组、LSIL组, LSIL组显著高于NC组,差异有统计学意义(P<0.05)。NC组的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA阳性率分别为25.00%、27.50%, LSIL组分别为76.00%、71.00%, HSIL组分别为95.00%、100.00%。三组的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA阳性率比较,差异有统计学意义(P<0.05);HSIL组的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA阳性率显著高于NC组、LSIL组,LSIL组显著高于NC组,差异有统计学意义(P<0.05)。LSIL组中不同转归者的宫颈脱落细胞中mybl2mRNA及HPV E6/E7 mRNA表达量、阳性率比较,差异有统计学意义(P<0.05);进展者的宫颈脱落细胞中mybl2 mRNA及HPV E6/E7 mRNA表达量、阳性率显著高于持续者和消退者,持续者显著高于消退者,差异有统计学意义(P<0.05)。Pearson相关性分析显示, LSIL患者宫颈脱落细胞中mybl2 mRNA与HPV E6/E7 mRNA呈正相关(r=0.816, P<0.05)。结论 LSIL患者宫颈脱落细胞中mybl2 mRNA的表达与HPV E6/E7 mRNA密切相关,其在LSIL患者中的分流价值较高。

Objective To explore the shunting value on the expression of cervical exfoliated cells mybl2 mRNA in low-grade squamous intraepithelial lesion(LSIL)patients.Methods 180 patients with cervical lesions were divided into cervicitis(NC)group(40 cases),LSIL group(100 cases)and high-grade squamous intraepithelial lesion(HSIL)group(40 cases)according to the pathological results.Comparison was made on the expression level and positive rates of cervical exfoliated cells mybl2 mRNA and human papillomavirus(HPV)E6/E7 mRNA among the three groups and among patients with different outcomes in LSIL group.The correlation between mybl2 mRNA and HPV E6/E7 mRNA in cervical exuded cells of LSIL patients was analyzed.Results The expression levels of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA of NC group were(1.23±0.16)and(1.76±0.32),respectively,those of LSIL group were(3.39±0.59)and(5.01±1.32),and those of HSIL group were(6.56±0.93)and(8.10±1.69).The expression levels of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA of the three groups were compared,and the difference was statistically significant(P<0.05).The expression levels of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA of HSIL group were significantly higher than those of NC group and LSIL group,and LSIL group was significantly higher than NC group.The difference was statistically significant(P<0.05).The positive rates of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA were 25.00%and 27.50%in NC group,76.00%and 71.00%in LSIL group,and 95.00%and 100.00% in HSIL group. The positive rates of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA of the three groups were compared, and the difference was statistically significant (P<0.05). The positive rates of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA in HSIL group was significantly higher than those in NC group and LSIL group, and the positive rates in LSIL group was significantly higher than those in NC group. The difference was statistically significant (P<0.05). The expression level and positive rates of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA of patients with different outcomes in LSIL group were compared, and the difference was statistically significant (P<0.05). The expression level and positive rates of cervical exfoliated cells mybl2 mRNA and HPV E6/E7 mRNA of patients with disease progression were significantly higher than those of patients with disease regression and persistence, and the expression level and positive rates of patients with disease persistence were significantly higher than those of patients with disease regression. The difference was statistically significant (P<0.05). Pearson correlation analysis showed that mybl2 mRNA was positively correlated with HPV E6/E7 mRNA in cervical exuded cells of LSIL patients (r=0.816, P<0.05). Conclusion The expression of cervical exfoliated cells mybl2 mRNA in LSIL patients is closely related to HPV E6/E7 mRNA, and its shunting value in LSIL patients is high.