探讨双歧杆菌四联活菌改善SAP大鼠炎症反应的效果

Effect of Bifidobacterium quadruple viable tablet on improving inflammation in SAP rats

目的探讨双歧杆菌四联活菌在改善重症急性胰腺炎(SAP)大鼠炎症反应中的治疗价值。方法健康wistar雄性大鼠共24只,按随机原则分为实验组、对照组及空白组,每组8只。实验组和对照组大鼠分别于造模前7 d及造模后24 h内给予双歧杆菌四联活菌片0.75 g,b.i.d.和玉米片0.75 g,b.i.d.。空白组大鼠同质饲料喂食,未行造模及手术处理。比较三组大鼠血清淀粉酶(AMY)、白细胞计数(WBC)、C反应蛋白(CRP)水平,观察大鼠胰腺组织病理损伤。结果与空白组血清AMY(2483.25±197.98)U/L相比,对照组以及实验组的血清AMY(3521.13±419.22)、(2859.13±136.56)U/L明显升高,实验组与对照组相比明显较低,差异均有显著统计学意义(P<0.01)。与空白组血清WBC(2.655±0.524)×10^(9)/L、CRP(4.889±0.489)mg/L相比,实验组的血清WBC(4.770±0.655)×10^(9)/L、CRP(5.840±0.311)mg/L与对照组血清WBC(6.590±0.755)×10^(9)/L、CRP(7.019±0.806)mg/L均明显升高,但与对照组相比,实验组经双歧杆菌四联活菌片干预后,血清WBC、CRP水平明显降低,差异均有显著统计学意义(P<0.01)。同时,实验组胰腺组织损伤程度、胰腺炎症细胞浸润程度、小叶间隙增宽及胰腺细胞坏死程度与对照组相比均减轻。结论应用双歧杆菌四联活菌对改善SAP大鼠胰腺损害程度及炎症反应程度有明显的治疗价值,可为临床减轻SAP患者炎症反应提供动物实验理论依据和参考靶点。

Objective To explore the value of Bifidobacterium quadruple viable tablet on improving inflammation in severe acute pancreatitis(SAP)rats.Methods 24 healthy wistar male rats were randomly divided into an experimental group,a control group and a blank group,and with 8 rats in each group.The rats in the experimental group and the control group were given Bifidobacterium quadruple viable tablet 0.75 g,b.i.d.,and corn tablets 0.75 g,b.i.d.7 d before modeling and 24 h after modeling respectively.The rats in blank group were fed the same diet without modeling or operation.The levels of serum amylase(AMY),white blood cell count(WBC)and C-reactive protein(CRP)in three groups were compared to observe the pathological injury of pancreatic tissue.Results The serum AMY of the control group and the experimental group were(3521.13±419.22)and(2859.13±136.56)U/L,which were obviously higher than(2483.25±197.98)U/L in the blank group;the experimental group was obviously lower than the control group;the difference was statistically significant(P<0.01).The experimental group had serum WBC of(4.770±0.655)×10^(9)/L and CRP of(5.840±0.311)mg/L,and the control group had serum WBC of(6.590±0.755)×10^(9)/L and CRP of(7.019±0.806)mg/L,which were obviously higher than(2.655±0.524)×10^(9)/L and(4.889±0.489)mg/L in blank group;however,compared with the control group,the serum levels of WBC and CRP in the experimental group were significantly reduced after the intervention of Bifidobacterium quadruple viable tablet,and the difference was statistically significant(P<0.01).At the same time,the degree of pancreatic tissue injury,the degree of pancreatic inflammatory cell infiltration,the widening of lobular space and the degree of pancreatic cell necrosis were reduced in the experimental group compared with the control group.Conclusion The application of Bifidobacterium quadruple viable tablet has obvious therapeutic value in improving the degree of pancreatic damage and inflammation in SAP rats,and can provide theoretical basis and reference target for reducing the inflammation in patients with severe acute pancreatitis clinically.