云南不同来源大发表中异荭草苷含量比较研究

Comparative Study on the Content of Isoorientin in Campylotropis Trigonoclada(Franch.) Schindl from Different Sources in Yunnan

目的 考察不同产地、部位、生长年限及采收期大发表药材中异荭草苷的含量变化,为大发表药材后续研究应用提供参考。方法 采用高效液相色谱法,测定云南大理、楚雄、玉溪3个大发表药材主要产地,以及玉溪产大发表药材的不同部位、生长年限及采收期的异荭草苷含量。结果 不同产地的大发表药材中异荭草苷含量存在差异,其中,玉溪产大发表药材的异荭草苷含量为190μg/g,较优于大理和楚雄;大发表药材的不同部位中异荭草苷含量存在显著差异,异荭草苷含量最高的是花,其次是叶、枝、茎、根等;从1年生到3年生,大发表药材中异荭草苷含量变化趋势不明显;从7月初到11月初,大发表药材中异荭草苷含量逐渐升高。结论 初步揭示了不同产地、部位、生长年限及采收期大发表药材中异荭草苷的含量变化情况,为大发表药材质量控制研究和推广应用提供参考。

Objective To investigate the content changes of isoorientin in different producing areas,parts,growth periods,and harvest periods,so to provide reference and application for the follow-up studies of Campylotropis trigonoclada(Franch.) Schindl.Methods The content of isoorientin in C.trigonoclada(Franch.) Schindl in three major producing areas of Dali,Chuxiong,and Yuxi of Yunnan province,as well as this plant of different parts,growth years,and harvest periods collected from Yuxi,were determined by HPLC.Results There are differences in the content of isoorientin in the Campylotropis trigonoclada(Franch.) Schindl from different producing areas.Among them,the content of isoorientin in C.trigonoclada(Franch.) Schindl produced in Yuxi is 190 μg/g,which is "better than those produced in Dali and Chuxiong.There are significant differences in the content of isoorientin in different parts of the Campylotropis trigonoclada(Franch.)Schindl.The content of isoorientin is the highest in flowers,followed by leaves,branches,main branches and roots.From 1-year-old to 3-year-old,the change trend of isoorientin content in the Campylotropis trigonoclada(Franch.) Schindl is not obvious;from early July to the beginning of November,the content of isoorientin in the Campylotropis trigonoclada(Franch.)Schindl increased gradually.Conclusion The content changes of isoorientin in different producing areas,parts,growth years and harvest period of C.trigonoclada(Franch.) Schindl were preliminarily revealed,which provided reference for the quality control research and popularization and application of C.trigonoclada.