赤嘴潜鸭真菌病组织病理学检查和病原分离鉴定

Histopathological Observation and Pathogen Identification of Mycosis Infection in Netta rufina

为了对动物园野禽饲养管理和疾病诊断提供参考,本试验对北京市野生动物园死亡的赤嘴潜鸭进行解剖、大体病变观察;分离死亡野鸭的气囊和肝脏,制作石蜡切片,经苏木素-伊红(H.E.)染色、过碘酸雪夫(PAS)染色和Masson染色后进行组织病理学检查;分离气囊病原培养后测序并进行BLAST比对分析。结果显示,死亡野鸭气囊增厚、气囊内充满真菌菌丝,肝脏纤维结缔组织增生;病原经鉴定为曲霉菌。结果表明,该野鸭死亡的原因为真菌感染。

To provide reference for zoo avian husbandry management and disease diagnosis,this study conducted autopsy and gross lesion observation on Netta rufina that died at Beijing Wildlife Park.Samples from the air sacs and liver of the deceased ducks were collected for histopathological examination through paraffin sectioning,hematoxylin-eosin(H.E.)staining,periodic acid-schiff(PAS)staining,and Masson staining.Pathogens from the air sacs were isolated,cultured,sequenced,and analyzed via BLAST comparison.The results revealed thickened air sacs filled with fungal hyphae and proliferative fibrous connective tissue in the liver.The identified pathogen was Aspergillus.The findings indicate that the cause of death was fungal infection.