分子生物学技术在食源性致病菌快速检测中的研究进展

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

食源性致病菌是影响食品安全的重要因素之一,高效快速的食源性致病菌检测技术对保障食品安全尤为重要。在食源性致病菌快速检测方法中,分子生物学技术因特异性强、精准度高、检测速度快等优势得到广泛应用。本文介绍了多种分子生物学技术在食源性致病菌快速检测中的应用,包括聚合酶链式反应(polymerase chain reaction,PCR)、环介导等温扩增(loop-mediated isothermal amplification,LAMP)、依赖核酸序列的扩增(nuclear acid sequence-based amplification,NAS-BA)、重组酶聚合酶扩增(recombinase polymerase amplification,RPA)、重组酶介导的等温核酸扩增(recombinase-aided isothermal amplification,RAA)、基因芯片(gene chip,GC),并对这些技术的应用现状、适用范围以及存在的问题等进行了分析总结,以期对未来的食源性致病菌快速检测技术提供理论参考。

Foodborne pathogens are one of the important factors affecting food safety.Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.Among the rapid detection methods of foodborne pathogens,molecular biology technology has been widely used because of its advantages of strong specificity,high accuracy,and fast detection speed.This paper introduces the application of various molecular biological techniques in the rapid detection of foodborne pathogens,including polymerase chain reaction(PCR),loop-mediated isothermal amplification(LAMP),nuclear acid sequence-based amplification(NAS-BA),recombinase polymerase amplification (PRA), recombinase-aided isothermal amplification (RAA), and gene chip (GC). The application status, application scope and existing problems of these technologies are also analyzed and summarized to provide theoretical reference for the rapid detection of foodborne pathogens in the future.