应用激光散斑衬比成像探究牙龈生物型对正畸加力下牙龈血流变化的影响

Effect of Gingival Biotype on Changes in Gingival Blood Flow under Orthodontic Forces Investigated by Laser Speckle Contrast Imaging

目的:应用激光散斑衬比成像(laser speckle contrast imaging,LSCI)探究牙龈生物型对正畸加力下牙龈血流变化的影响。方法:招募24例符合纳入标准的受试者参与研究,包括薄龈型组12例,厚龈型组12例,随机在每例受试者上颌左侧或右侧的尖牙至第一磨牙间施加150 g的力,并在施力前、施力30 min、1 d、3 d、7 d、14 d分别使用LSCI动态监测施力侧尖牙区牙龈血流灌注。结果:组间比较,薄龈型组与厚龈型组各时间点的血流值比较均存在显著差异,厚龈型血流值显著高于薄龈型(P<0.05);组内比较,每组各时间点的血流值比较也存在显著差异(P<0.05),施力后,局部血流灌注在30 min~1 d内降至最小值,7 d左右上升至最大值,14 d时基本恢复到基线。在没有力值加载的静息状态下,游离龈与牙间乳头的血流值比较存在显著差异(P<0.05),游离龈血流值显著低于牙间乳头。结论:LSCI是正畸加力过程中实时监测牙龈血流灌注的良好工具,薄龈型血液供应显著低于厚龈型。

Objective:To explore the effect of gingival biotype on gingival blood flow(BF)changes under orthodontic forces by laser speckle contrast imaging(LSCI).Methods:The study recruited a total of 24 subjects who met the inclusion criteria,with 12 individuals allocated to the thin gingival group and another 12 allocated to the thick gingival group.A force of 150 g was randomly applied between either the left or right maxillary canines and the first molar in each volunteer.Subsequently,dynamic monitoring of BF using LSCI was conducted at various time points:before force application,at 30 minutes,1 day,3 days,7 days,and 14 days.Results:Through inter group comparison,there were significant differences in BF between the thin group and the thick group at various time points,with the BF of the thick group significantly higher than that of the thin group(P<0.05).After intra group comparison,there were significant differences in BF at each time point in each group(P<0.05).After applying force,local BF decreased to the minimum value within 30 minutes to 1 day,rose to the maximum value around 7 days,and basically returned to the baseline by 14 days.In a resting state without force loading,there was a significant difference in BF between the free gingiva and interdental papilla(P<0.05),and the BF of the free gingiva was significantly lower than that of the interdental papilla area.Conclusion:LSCI is a useful tool to real-time monitor gingival BF during orthodontic treatment.The BF of thin gingival type was significantly lower than that of thick gingival type.