致敏红细胞BPGM酶活性降低对红细胞携氧/释氧能力的影响研究

The Impact of Reduced Bisphosphoglycerate Mutase(BPGM)Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity

目的 分析致敏红细胞的携氧/释氧能力并初步探讨出现异常的原因。方法 用微柱凝胶卡检测致敏红细胞,用携氧/释氧功能评价装置分析自身免疫性溶血性贫血(AIHA)和新生儿溶血病(HDN)患者的致敏红细胞的携氧/释氧能力。使用健康成人的血浆构建输血性溶血反应小鼠模型,对照组给予PBS,记录小鼠负重游泳至力竭的时间,分离小鼠红细胞并分析其携氧/释氧能力,利用蛋白组学对小鼠溶血组与正常组血浆中差异蛋白进行GO和KEGG富集分析。采用ELISA方法检测致敏红细胞BPGM酶活性和2,3-DPG含量。结果 与相应对照组相比,AIHA、HDN和输血性溶血反应模型小鼠的红细胞的氧解离曲线均发生左移和P50下降。溶血模型小鼠的负重游泳时间明显缩短,蛋白组学筛选结果发现红细胞BPGM异常,致敏红细胞BPGM酶活性降低且细胞内2,3-DPG含量下降。结论 致敏红细胞BPGM酶活性降低导致红细胞释放氧气能力下降。

Objective To explore the underlying causes for the observed anomalies,the oxygen-carrying/releasing capacity of sensitized red blood cells(RBCs)was analyzed.Methods The sensitized red blood cells were detected through microcolumn gel cards,and an oxygen-carrying/releasing assessment device was employed to evaluate the oxygen-carrying/releasing capacity of sensitized RBCs in patients with autoimmune hemolytic anemia(AIHA)and hemolytic disease of the newborn(HDN).We constructed a transfusion-induced hemolytic reaction mouse model using plasma from healthy adults,while the control group received PBS.Record the time until exhaustion during weight-loaded swimming in mice and analyze the oxygen-carrying/releasing capacity of isolated mouse RBCs.Perform GO and KEGG enrichment analysis on differentially expressed proteins related to hemolysis and the control group using proteomics.Measure BPGM enzyme activity and 2,3-DPG levels in sensitized RBCs using ELISA.Results Compared to the corresponding control groups,the oxygen dissociation curves in AIHA,HDN,and transfusion-induced hemolytic reaction model mice exhibited a left shift and decreased P50.The weightloaded swimming time in hemolytic model mice was significantly shortened.Proteomic screening revealed abnormalities in BPGM,with decreased BPGM enzyme activity and a decline in intracellular 2,3-DPG content.Conclusion The reduced activity of BPGM in sensitized RBCs led to a decline in their oxygen-releasing capacity.