摘要
【目的】基于转录组测序分析溶解氧对四指马鲅(Eleutheronema tetradactylum)幼鱼鳃组织的影响,探究四指马鲅应对低氧胁迫的适应机制,为四指马鲅的抗逆性研究提供参考依据。【方法】以四指马鲅幼鱼为试验材料,设3种溶解氧含量处理,常氧组(CG)6.33±0.15mg/L、中度低氧组(OC4)3.99±0.18mg/L和重度低氧组(OC2)2.05±0.15mg/L,处理29 h,采集鳃组织进行转录组测序,利用DEGseq筛选不同比较组的差异表达基因(DEGs),使用GOseq进行GO功能注释分析,利用KOBAS进行KEGG信号通路富集分析,采用STEM对DEGs进行表达趋势分析。【结果】OC4 vs CG比较组有86个DEGs,其中28个上调表达,58个下调表达;OC2 vs CG比较组有2018个DEGs,其中740个上调表达,1278个下调表达;OC4vsOC2比较组有171个DEGs,其中68个上调表达,103个下调表达。GO功能注释分析结果显示,DEGs主要涉及代谢过程、有机物生物合成、细胞氧化还原平衡、细胞内、染色质、核糖体结构成分和催化活性等功能条目。KEGG信号通路富集分析结果显示,DEGs主要富集在蛋白酶体、DNA复制、脂肪酸降解、磷酸戊糖途径、氧化磷酸化、谷胱甘肽代谢、赖氨酸降解和核糖体生物发生等信号通路;其中pac1、lsm7、mcm6、mcm2和mdm2等基因富集在蛋白酶体、剪接体、DNA复制和细胞周期等信号通路,与四指马鲅幼鱼鳃组织蛋白合成有关,随着溶解氧含量下降,其表达量呈下降趋势;shmt2、hsd17b10、sucla2和glo1等基因富集在磷酸戊糖途径信号通路,与四指马鲅幼鱼鳃组织能量代谢有关。【结论】四指马鲅幼鱼鳃组织蛋白质合成能力和能量代谢活动均随着溶解氧含量下降而下降,溶解氧含量下降显著影响细胞周期、DNA复制、脂肪酸降解和糖代谢等信号通路。
【Objective】Transcriptome sequencing-based analysis of the effects of dissolved oxygen on gill tissues of juvenile Eleutheronema tetradactylum was conducted to investigate the adaptive mechanisms of E.tetradactylum in response to hypoxia stress.To provide a reference basis for research on the resistance of E.tetradactylum.【Method】Juvenile E.tetradactylum were used as experimental materials,and three dissolved oxygen content treatments were set up,normoxic group(CG)6.33±0.15 mg/L,moderately hypoxic group(OC4)3.99±0.18 mg/L,and severely hypoxic group(OC2)2.05±0.15 mg/L.The treatments were carried out for 29 h,and gill tissues were collected for transcriptome sequencing.Differentially expressed genes(DEGs)from different comparison groups were screened using DEGseq,GO functional annotation analysis was performed using GOseq,and KEGG signaling pathway enrichment analysis was performed using KOBAS,trends of DEGs were analyzed by STEM.【Result】The OC4 vs CG comparison group had 86 DEGs,of which 28 were up-regulated and 58 were down-regulated;the OC2 vs CG comparison group had 2018 DEGs,of which 740 were up-regulated and 1278 were down-regulated;and the OC4 vs OC2 comparison group had 171 DEGs,of which 68 were up-regulated and 103 were down-regulated.The results of GO functional annotation analysis showed that DEGs were mainly involved in functional terms such as metabolic processes,organic matter biosynthesis,cellular redox homeostasis,intracellular,chromatin,ribosomal structural components and catalytic activity.KEGG signaling pathway enrichment analysis showed that DEGs were mainly enriched in signaling pathways such as proteasome,DNA replication,fatty acid degradation,pentose phosphate pathway,oxidative phosphorylation,glutathione metabolism,lysine degradation,and ribosome biogenesis;among them,genes such as pac1,lsm7,mcm6,mcm2 and mdm2 were enriched in signaling pathways such as proteasome,spliceosome,DNA replication and cell cycle,they were related to protein synthesis in gill tissues of juvenile E.tetradactylum,and the expression of which showed a decreasing trend with the decrease of dissolved oxygen content;shmt2,hsd17b10,sucla2 and glo1 genes were enriched in pentose phosphate pathway,which were related to energy metabolism in gill tissues of juvenile E.tetradactylum.【Conclusion】Both protein synthesis capacity and energy metabolism activity of gill tissues of juvenile E.tetradactylum decrease with decreasing dissolved oxygen content,decrease of dissolved oxygen content significantly affects signaling pathways such as cell cycle,DNA replication,fatty acid degradation and sugar metabolism.
作者
林欣
谢希尧
区又君
李加儿
LIN Xin;XIE Xi-yao;OU You-jun;LI Jia-er(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou,Guangdong 510300,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian,Liaoning 116023,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2024年第3期660-669,共10页
Journal of Southern Agriculture
基金
国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0900200)
广东省乡村振兴战略专项资金种业振兴项目(2022-SPY-00-013)
中国水产科学研究院基本科研业务费专项(2023TD58)。
关键词
四指马鲅
幼鱼
鳃
溶解氧
转录组
Eleutheronema tetradactylum
juvenile fish
gill
dissolved oxygen
transcriptome