基于化学计量学对柑橘属果实类药材间的差异性研究

Study on the difference between Citrus fruit medicinal materials based on chemometrics

目的建立同时测定芸香柚皮苷、柚皮苷、橙皮苷、野漆树苷、新橙皮苷5种黄酮类成分的HPLC分析方法,对柑橘属5味果实类药材共82批进行差异性分析。方法采用Dikma Platisil ODS(5μm,250 mm×4.6 mm)色谱柱,以乙腈-0.1%甲酸水(体积比15∶85)为流动相,检测波长为283 nm,流速为1.0 mL/min,柱温为35℃;采用化学计量学的方法对柑橘属5味果实类药材间的差异进行研究。结果5种化学成分线性关系良好(r>0.9990),平均加样回收率为98.37%~103.24%,精密度、稳定性、重复性试验RSD值均小于3%。统计学分析表明5种黄酮类成分的质量分数在5味药材间差异均具有统计学意义;化学计量学分析表明新橙皮苷、橙皮苷、柚皮苷是5味药材的差异性成分;新橙皮苷是酸橙枳壳及酸橙枳实、枳壳基原、枳实基原鉴别的差异性成分。结论建立了简便可行、精密稳定的柑橘属类药材的多指标质量分数测定方法,可为柑橘属果实类药材的基原鉴定、质量评价及临床应用提供参考。

Objective To establish an HPLC analysis method for the simultaneous determination of 5 flavonoids include narirutin,naringin,hesperidin,rhoifolin and neohesperidin,and analyze the differences of 82 batches of the five Citrus fruits medicinal materials.Methods DIKMA Platisil column(4.6 mm×250 mm,5μm)was used,mobile phase was consisted of acetonitrile-0.1%formic acid(15∶85),the detection wavelength was 238 nm,the flow rate was 1.0 mL/min,and the column temperature was 35℃.The differences between species of Citrus fruits medicinal were studied by analysis of variance and chemometrics.Results The results showed that the determination of 5 flavonoids manifested a good liner relationship(r>0.9990),and the average recovery rate was 98.37%-103.24%.The relative standard deviations(RSDs)of precision,stability,and repeatability were all less than 3.0%.Statistical analysis showed that the content of the five flavonoids was significantly different between the five medicinal materials.Chemometrics showed that neohesperidin,hesperidin and naringin were the differential components of the five medicinal materials.Neohesperidin were the main differential components of Aurantii Fructus and Aurantii Fructus Immaturus of Citrus Aurantium,Aurantii Fructus and Aurantii Fructus Immaturus of different origins.Conclusion This study has established a simple,feasible,precise and stable multi-index content determination method for Citrus medicinal materials,which can provide reference for the identification,quality evaluation and clinical application of citrus fruit medicinal materials.