基于斑马鱼模型的黄芩提取物抗炎和抗氧化活性评价

Evaluation of anti-inflammatory and antioxidant activities of Scutellaria baicalensis extracts based on zebrafish modelin

目的采用斑马鱼模型研究黄芩提取物毒性、抗炎作用和抗氧化作用。方法采用截断转基因斑马鱼Tg(colo1a:EGFp)的尾鳍构建损伤炎症模型,荧光显微镜下观察绿色荧光蛋白(EGFp)标记的中性粒细胞和巨噬细胞数量,评价黄芩提取物抗炎症细胞聚集和反向迁移能力;采用LPS诱导构建氧化应激模型,DCFH-DA荧光探针染色斑马鱼体内活性氧(ROS)含量水平评价黄芩提取物对斑马鱼的抗氧化作用。结果黄芩提取物在质量浓度为0.60和1.25 mg/mL均能抑制炎症细胞聚集和促进炎症细胞清除,且呈现剂量依赖效应;黄芩提取物在质量浓度为0.02、0.04 mg/mL均具有减弱斑马鱼ROS相对荧光强度的作用,且呈剂量依赖效应。结论黄芩提取物具有明显的抗炎和抗氧化作用。

Objective To explore the toxicity,anti-inflammatory and antioxidant effects of Scutellaria baicalensis extract by using zebrafish models.Methods An injury inflammation model was constructed by truncating the caudal fin of transgenic zebrafish Tg(colo1a:EGFp).The number of green fluorescent protein(EGFp)-labeled neutrophils and macrophages was observed under fluorescence microscope to evaluate the anti-inflammatory cell aggregation and reverse migration ability of Scutellaria baicalensis extract.Using LPS induction to construct an oxidative stress model,DCFH-DA fluorescent probe was used to stain the levels of reactive oxygen species(ROS)in zebrafish to evaluate the antioxidant effect of Scutellaria baicalensis extract on zebrafish.Results Scutellaria baicalensis extract at concentrations of 0.60 and 1.25 mg/mL both inhibited inflammatory cell aggregation and promoted inflammatory cell clearance in a dose-dependent manner.Scutellaria baicalensis extract at concentrations of 0.020 and 0.040 mg/mL reduced the relative fluorescence intensity of zebrafish ROS in a dose-dependent manner.Conclusion Scutellaria baicalensis extract has a notable anti-inflammatory and antioxidant effect.