萆薢渗湿汤对急性湿疹小鼠模型Th1/Th2/Th22免疫功能的影响

Effect of Bixie Dampness-excreting Decoction on Th1/Th2/Th22 immune function in mice model with acute eczema

目的:探讨萆薢渗湿汤对急性湿疹小鼠模型的治疗效果及对Th细胞介导的免疫功能相关机制的研究。方法:将30只KM小鼠随机分为空白组(Normal)、模型组(Model)和萆薢渗湿汤治疗组(BXSST),采用2,4-二硝基氯苯(DNCB)建立急性湿疹小鼠模型。造模成功后,治疗组给予1.3 g/ml萆薢渗湿汤0.2 ml/只灌胃,空白组与模型组给予等量生理盐水灌胃。HE染色观察小鼠耳部组织的病理变化,ELISA检测小鼠血清中白细胞介素22(IL-22)、白细胞介素-4(IL-4)和白细胞介素-2(IL-2)的表达水平。结果:HE染色病理切片显示与模型组比较,萆薢渗湿汤组表皮棘层厚度明显降低,组织内炎症细胞浸润减少;与空白组相比,模型组小鼠血清中IL-2表达降低(P<0.01),IL-4、IL-22水平均明显升高(P<0.01);与模型组比较,萆薢渗湿汤治疗组中IL-2表达升高(P<0.01),IL-4与IL-22水平降低(P<0.05)。结论:萆薢渗湿汤可通过调节IL-2、IL-4及IL-22细胞因子的水平,来改善小鼠急性湿疹的症状。

Objective:To investigate the therapeutic effect of Bixie Dampness-excreting Decoction on acute eczema mice model and the related mechanism of Th cell-mediated immune function.Methods:Thirty KM mice were randomly divided into normal group,model group and BXSST treatment group.The mice model of acute eczema was established by 2,4-dinitrochlorobenzene(DNCB).After the model was successfully established,the mice in the treatment group were given 1.3 g/ml Bixie Dampness-excreting Decoction by gavage,0.2 ml/one mouse;and the mice in the normal group and the model group were given the same amount of normal saline by gavage.The pathological changes of ear tissues of mice were observed by HE staining.ELISA was used to detect the expression levels of interleukin-22(IL-22),interleukin-4(IL-4)and interleukin-2(IL-2)in serum of mice.Results:HE stained pathological sections showed that compared with the model group,the thickness of epidermal spinous layer in the BXSST treatment group was significantly reduced,and the infiltration of inflammatory cells in the tissue was reduced.Compared with normal group,the expression of IL-2 in serum in model group was decreased(P<0.01),and the levels of IL-4 and IL-22 were significantly increased(P<0.01).Compared with the model group,the expression of IL-2 in the BXSST treatment group was increased(P<0.01),and the levels of IL-4 and IL-22 were decreased(P<0.05).Conclusion:Bixie Dampness-excreting Decoction can improve the symptoms of acute eczema in mice by regulating the levels of IL-2,IL-4 and IL-22 cytokines.