罗哌卡因对老年大鼠胫骨骨折术后认知功能障碍和神经突触的影响

Effects of ropivacaine in cognitive dysfunction and synapses after tibial fracture in aged rats

目的研究罗哌卡因对老年大鼠胫骨骨折术后认知功能障碍和神经突触的影响。方法将SD雄性大鼠分为假手术组、模型组和低、中、高剂量实验组。假手术组大鼠在局部麻醉下切开皮肤并缝合,其余4组进行开放性胫骨骨折手术,其中假手术组、模型组均给予七氟烷麻醉,低、中、高剂量实验组在七氟烷麻醉基础上给予0.5、1.0、2.0 mg·kg^(-1)罗哌卡因。术后7 d各组大鼠进行旷场实验和Morris水迷宫实验,评估Longa评分,用免疫组化法检测大鼠海马组织突触素1(Syn1)水平,用蛋白质印迹法检测N-甲基-D-天冬氨酸受体2B(NMDAR2B)、钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)和Syn1蛋白的表达水平。结果假手术组、模型组和低、中、高剂量实验组Longa评分分别为0、(3.50±0.71)、(2.80±0.63)、(2.20±0.63)和(0.90±0.32)分,Syn1的累积光密度分别为0.56±0.09、0.25±0.03、0.34±0.03、0.42±0.03和0.50±0.05,Syn1蛋白相对表达水平分别为1.08±0.12、0.42±0.05、0.55±0.07、0.72±0.06和0.86±0.05,NMDAR2B蛋白相对表达水平分别为1.28±0.13、0.51±0.07、0.69±0.06、0.84±0.07和1.02±0.11,CaMKⅡ蛋白相对表达水平分别为0.94±0.08、0.36±0.04、0.50±0.06、0.71±0.06和0.86±0.06。假手术组与模型组的上述指标比较,在统计学上差异均有统计学意义(均P<0.05);模型组与低、中、高剂量实验组的上述指标比较,在统计学上差异均有统计学意义(均P<0.05)。结论罗哌卡因可改善老年大鼠胫骨骨折术后认知功能障碍和神经突触。

Objective To explore the effects of ropivacaine on cognitive dysfunction and synapses in aged rats after tibial fracture.MethodSsSD male rats were divided into sham operation group,model group and low,medium,high dose experimental groups.Sham operation group was incised and sutured under local anesthesia,and other four groups underwent open tibial fracture operation.Sham operation group and model group were given sevoflurane anesthesia,low,medium and high dose experimental groups were given 0.5,1.0,2.0 mg·kg^(-1)ropivacaine on the basis of sevoflurane anesthesia.Open field test and Morris water maze test were performed 7 days after operation;Longa score was evaluated;neurotransmitter levels were detected by kit;and Synl in hippocampus of rats was detected by immunohistochemistry Western blot analysis was used to detected the expression of N-methyl-D-aspartate receptor 2B(NMDAR2B),calmodulin-dependent protein kinase Ⅱ(CaMK Ⅱ)and Synl.Results The Longa scores of sham operation group,model group and low,medium,high dose experimental groups were 0,(3.50±0.71),(2.80±0.63),(2.20±0.63)and(0.90±0.32)points;the integrated optical density of Synl were 0.56±0.09,0.25±0.03,0.34±0.03,0.42±0.03 and 0.50±0.05;the expression of Synl protein were 1.08±0.12,0.42±0.05,0.55±0.07,0.72±0.06 and 0.86±0.05;the expression of NMDAR2B protein were 1.28±0.13,0.51±0.07,0.69±0.06,0.84±0.07 and 1.02±0.11;CaMK Ⅱ protein were 0.94±0.08,0.36±0.04,0.50±0.06,0.71±0.06 and 0.86±0.06.There were statistically significant differences between sham operation group and model group(P<0.05);there were significant differences in the above indexes between model group and low,medium,high dose experimental groups(all P<0.05).Conclusion Ropivacaine can improve cognitive dysfunction and synapses in aged rats after tibial fracture.