抑制宿主脂肪酸合成关键酶活性影响O型口蹄疫病毒的复制

Effect on FMDV type O replication by inhibition of host fatty acid synthesis key enzymes activities

为探究宿主脂肪酸合成对O型口蹄疫病毒(FMDV)复制的影响,以猪肾细胞PK-15和IBRS-2细胞为模型,监测脂肪酸合成的关键酶抑制剂对FMDV复制的调控作用;通过CCK-8试剂盒检测抑制剂对细胞的毒性作用,筛选合适的抑制剂预处理浓度;用Western-blot和实时荧光定量PCR(qPCR)方法检测添加外源C75和TOFA对FMDV复制的调控作用。结果显示,和对照组相比,添加脂肪酸合成的关键酶抑制剂C75和TOFA后,口蹄疫病毒蛋白水平和转录水平均显著下调。结论,抑制宿主细胞脂肪酸合成会降低FMDV在宿主细胞中的复制效率,这为进一步深入研究FMDV的致病机制开辟了新的方向。

In order to investigate the effect of fatty-acid biosynthesis in host cells on the replication of foot-and-mouth disease virus type O,the PK-15 cells and the IBRS-2 cells as cell models,respectively,were used to explore the regulation of FMDV replication.The toxic effects of inhibitors on cells were detected by CCK-8 kit to screen the appropriate pretreatment concentration of the inhibitors.Western-blot and real-time fluorescence quantification(qPCR)were used to detect the regulatory effects of exogenous addition of C75 and TOFA on FMDV replication.The results showed that,compared with the control group,the protein level and transcript level of FMD were significantly down-regulated after the adding TOFA or C75.In conclusion,inhibition of fatty acid synthesis key enzymes activities in host cells can reduce efficiency of FMDV replication,which broke a new path for further research on the pathogenic mechanism of FMDV.