脓毒症患儿外周血T淋巴细胞减数分裂重组11同源物A表达水平变化研究

The expression level of meiotic recombination 11 homolog A in peripheral blood T lymphocytes of children with sepsis

目的分析脓毒症患儿T淋巴细胞的减数分裂重组11同源物A(MRE11A)表达,初步探讨MRE11A对脓毒症T淋巴细胞功能的影响。方法选取2021年12月至2022年4月广西医科大学第一附属医院PICU住院的脓毒症患儿24例作为脓毒症组;同期本院门诊体检的24例健康儿童作为对照组。收集两组患儿性别、年龄,脓毒症组收集有创机械通气时间、实验室检查指标及住院时间等,采集入住PICU 24 h内外周血标本,对照组在研究期间同期采集外周血。采用流式细胞技术检测两组外周血T淋巴细胞胞质MRE11A,对比两组之间MRE11A表达阳性的细胞比例和表达量(用平均荧光强度表示)。结果脓毒症组、对照组各24例;脓毒症组男11例,女13例,中位年龄32.50(19.00,132.75)个月;对照组男14例,女10例,中位年龄57.50(33.75,102.25)个月;两组年龄和性别比较差异无统计学意义(均P>0.05)。脓毒症组MRE11A+CD4^(+)T淋巴细胞比例低于对照组[3.59(2.29,8.87)%比99.90(99.03,100.00)%,Z=-5.961,P<0.001],CD4^(+)T淋巴细胞的MRE11A表达量低于对照组[1663.00(1557.00,1777.00)比5689.00(4415.25,9567.00),Z=-5.939,P<0.001];脓毒症组MRE11A^(+)CD8^(+)T淋巴细胞比例低于对照组[3.85(1.87,9.58)%比92.15(76.70,96.40)%,Z=-5.856,P<0.001],CD8^(+)T淋巴细胞的MRE11A表达量低于对照组[1921.50(1680.00,2217.00)比4165.00(2894.00,9122.00),Z=-5.083,P<0.001]。结论MRE11A在脓毒症患儿T淋巴细胞胞质表达降低,可能影响了T淋巴细胞功能。

Objective To analyze the expression of meiotic recombination 1l homolog A(MRE11A)in T lymphocytes of children with sepsis,and to explore the effect of MRE11A on the function of T lymphocytes in sepsis.Methods Twenty-four children with sepsis hospitalized in PICU at the First Affiliated Hospital of Guangxi Medical University from December 2021 to April 2022 were selected as sepsis group.During the same period,24 healthy children who underwent outpatient physical examination in our hospital were selected as control group.The gender and age of two groups were collected,and the duration of invasive mechanical ventilation,laboratory examination index and hospitalization time were collected in sepsis group.The peripheral blood samples of sepsis group were collected within 24 hours of admission to PICU,and peripheral blood samples of control group were collected during the study period.Flow cytometry was used to detect the cytoplasmic MRE11A of peripheral blood T lymphocytes in two groups,and the proportion as well as expression of MREl1A positive cells between two groups were compared(expressed by mean fluorescence intensity).Results There were 24 cases in sepsis group,including 11 males and 13 females,with a median age of 32.50(19.00,132.75)months.There were 24 cases in control group,including 14 males and 10 females,with a median age of 57.50(33.75,102.25)months.There were no significant differences in gender and age between two groups(all P>0.05).The proportion of MRE11A^(+)CD4^(+)T lymphocytes in sepsis group was lower than that in control group[3.59(2.29,8.87)%vs.99.90(99.03,100.00)%,Z=-5.961,P<0.001].The expression of MRE11A in CD4^(+)T lymphocytes in sepsis group was lower than that in control group[1663.00(1557.00,1777.00)vs.5689.00(4415.25,9567.00),Z=-5.939,P<0.001].The proportion of MRE11A^(+)CD8^(+)T lymphocytes in sepsis group was lower than that in control group[3.85(1.87,9.58)%vs.92.15(76.70,96.40)%,Z=-5.856,P<0.001].The expression of MRE11A in CD8^(+)T lymphocytes in sepsis group was lower than that in control group[1921.50(1680.00,2217.00)vs.4165.00(2894.00,9122.00),Z=-5.083,P<0.001].Conclusion The expression level of MRE11A in the cytoplasm of T lymphocytes in children with sepsis was decreased,which may affect the function ofT lymphocytes.