车前子壳生物酶法制备阿拉伯低聚木糖水解条件优化

Optimization of Hydrolysis Conditions for Enzymatic Preparation of Arabinoxylo-Oligosaccharides by Psyllium Husk

车前子壳包含丰富的阿拉伯木聚糖,经木聚糖酶与阿拉伯糖苷酶的协同水解即可制备阿拉伯低聚木糖,相较于传统低聚木糖生产工艺,充分体现绿色制造优势。文章以车前子壳中阿拉伯木聚糖为底物,利用实验室自产木聚糖酶和阿拉伯糖苷酶复合酶液,采用单因素优化和响应面分析相结合的方式,对阿拉伯低聚木糖制备条件进行优化。单因素优化结果显示,当酶水解时间为5 d、阿拉伯木聚糖添加浓度为15 g/L、木聚糖酶添加量为100 IU/g阿拉伯木聚糖、水解液pH值为4.8、酶水解温度为50℃时,阿拉伯低聚木糖得率达到最高值,为(23.18±0.87)%。此外,利用响应面探讨阿拉伯木聚糖添加浓度、木聚糖酶添加量和酶水解温度三者间交互作用,最终确定酶水解条件:阿拉伯木聚糖添加浓度为13.90 g/L、木聚糖酶添加量为101.35 IU/g-阿拉伯木聚糖、酶水解温度为49.70℃,此时阿拉伯低聚木糖得率达到最优值(23.67±0.27)%,经验证试验证实模型预测精确度较高。

Psyllium husk contains abundant arabinoxylan,which can be hydrolyzed by xylanase and arabinosidase to prepare arabinoxylo-oligosaccharides.Compared with traditional oligosaccharide production processes,it fully embodies the advantages of green manufacturing.The article uses arabinoxylan in psyllium husk as the substrate,and uses a laboratory produced xylanase and arabinosidase composite enzyme solution.The preparation conditions of arabinoxylo-oligosaccharides are optimized using a combination of single factor optimization and response surface analysis.The single factor optimization results showed that when the enzyme hydrolysis time was 5 days,the concentration of arabinoxylan added was 15 g/L,the amount of xylanase added was 100 IU/g arabinoxylan,the pH value of the hydrolysis solution was 4.8,and the enzyme hydrolysis temperature was 50℃,the yield of arabinoxylo-oligosaccharides reached the highest value,which was(23.18±0.87)%.In addition,response surface methodology was used to explore the interaction between the concentration of arabinoxylan,the amount of xylanase added,and the enzyme hydrolysis temperature.Finally,the enzyme hydrolysis conditions were determined:when the concentration of arabinoxylan added was 13.90 g/L,the amount of xylanase added was 101.35 IU/g arabinoxylan,and the enzyme hydrolysis temperature was 49.70℃,the yield of arabinoxylo-oligosaccharides reached the optimal value(23.67±0.27)%.Verified experiments confirmed that the model's prediction accuracy was high.