枳椇果梗黄酮的提取及其抗氧化性

Extraction and Antioxidant Activity of Flavone from Hovenia dulcis Peduncle

为获得枳椇(Hovenia dulcis)果梗黄酮并对所得黄酮的抗氧化性进行评价,以枳椇果梗为原料,采用单因素结合响应面优化得到枳椇果梗黄酮的提取条件;经AB-8树脂分离纯化、真空旋转蒸发浓缩、冷冻干燥后得到枳椇果梗黄酮;并对该黄酮的自由基清除能力进行测定,同时以牛血清蛋白、亚油酸和鲱鱼精子DNA为对象构建体外蛋白质、脂质、DNA的氧化体系,测定其对生物大分子氧化的抑制效果。结果表明:枳椇果梗黄酮的最佳提取条件为料液比1:30(g/mL)、温度30℃、时间60 min、乙醇体积分数80%,在该条件下黄酮的得率为(3.17±0.03)%,纯度为66.67%;该黄酮对1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基、2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除的IC_(50)分别为(191.6±15.37)、(176.5±17.25)μg/mL,且对牛血清蛋白羰基化、亚油酸和鲱鱼精子DNA氧化均有较好的抑制作用,说明所得黄酮具有良好的抗氧化性。研究结果可为枳椇果梗黄酮的提取及抗氧化性研究提供一定的参考。

In order to obtain the Hovenia dulcis peduncle flavone and evaluate its antioxidant activity,Hovenia dulcis peduncle was used as raw material to optimize the extraction conditions of Hovenia dulcis peduncle flavone by single factor and response surface.The Hovenia dulcis peduncle flavone was purified by AB-8 resin,concentrated by vacuum rotation evaporation and freeze-dried,then its free radical scavenging ability of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation was determined.The oxidative system of protein,lipid and DNA in vitro was constructed with bovine serum protein,linoleic acid and herring sperm DNA as objects,and the oxidative inhibition ability of Hovenia dulcis peduncle flavone to biological macromolecules was evaluated.The results showed that the optimal extraction conditions were solid-liquid ratio 1:30(g/mL),temperature 30℃,time 60 min and ethanol concentration 80%,and the yield of flavone was(3.17±0.03)%,the purity is 66.67%.The IC_(50)of DPPH and ABTS free radical scavenging were(191.6±15.37)μg/mL and(176.5±17.25)μg/mL,respectively.And the flavone had effectively inhibition on the carbonylation of bovine serum protein,linoleic acid and herring sperm DNA oxidation,indicating the obtained flavone had good antioxidant activity.The results could provide a reference for the extraction of Hovenia dulcis peduncle flavone and its antioxidant activity study.