ZEB1-3′UTR促进乳腺癌细胞MCF-7迁移机制的研究

Study on the Mechanism of Zeb1-3′UTR Promoting the Migration of Breast Cancer Cells Mcf-7

对与ZEB1-3′UTR结合的microR-NAs进行生物信息学分析。结果表明,与对照组相比,过表达ZEB1-3′UTR后MCF-7细胞迁移能力显著增强。采用RNA22、Microrna、Targetscan数据库筛选出与ZEB1-3′UTR结合的microRNAs,三个数据库公共预测的microRNAs有9个,分别是miR-429、miR-200b、miR-200c、miR-494、miR-873、miR-381、miR-300、miR-431和miR-342,其中miR-429、miR-200b、miR-200c、miR-342在乳腺癌中高表达。以上研究表明,ZEB1-3′UTR能够竞争性结合肿瘤细胞表达的内源性microRNAs,促进了MCF-7细胞迁移。

The combination of ZEB1-3′UTR and microRNAs was analyzed by bioinformatics.Human breast cancer cell line MCF-7 was transfected with ZEB1-3′UTR expression vector,and the migration ability of breast cancer cells was ana-lyzed by Wound healing assay and transwell assay.The database RNA22,Microrna and Targetscan were used to predict the binding microRNAs of ZEB1-3′UTR,and the expression of these microRNAs in breast cancer was analyzed by UALCAN website.The results showed that compared with the control group,the migration ability of MCF-7cells was significantly enhanced after overexpression of ZEB1-3′UTR.The mi-croRNAs that binding to ZEB1-3′UTR were screened out by RNA22,Microrna and Targetscan database.Nine microRNAs highly expressed in breast cancer were predicted by three databases,namely miR-429,miR-200b,miR-200c,miR-494,miR-873,miR-381,miR-300,miR-431and miR-342.Among them,miR-429,miR-200b,miR-200c and miR-342.The research showed that ZEB1-3′UTR competitively binded to endogenous microRNAs expressed by tumor cells,and further promoted the migration of MCF-7cells.