鱼腥草中产槲皮素内生真菌的分离鉴定

Isolation and identification of endophytic fungi producingquercetin from Houttuynia cordata Thunb.

【目的】探索鱼腥草(Houttuynia cordata Thunb.)中是否存在产槲皮素的内生真菌。【方法】采用组织块法对鱼腥草内生真菌进行分离培养,分离纯化后收集菌丝利用薄层色谱(TLC)和高效液相色谱(HPLC)检测其代谢产物中的槲皮素,并分析其产量。采用形态学和内部转录间隔区(internal transcribed spacer,ITS)序列分析鉴定产槲皮素的内生真菌。【结果】从鱼腥草不同部位共分离得到9株内生真菌,其中由根中分离得到的菌株YXC-G1具有产生槲皮素的能力,为笋顶孢属(Acrostalagmus sp.)真菌,菌丝中槲皮素的含量为8.296 mg/g。【结论】从鱼腥草根部分离到1株产槲皮素的内生真菌YXC-G1,其可作为生产槲皮素的备选菌株。

【Objective】This study explored the presence of quercetin-producing endophytic fungi in Houttuynia cordata Thunb.【Method】The endophytic fungi in Houttuynia cordata Thunb.were isolated and cultured by tissue block method.After separation and purification,the mycelia were collected to detect quercetin in their metabolites by TLC and HPLC,and the yield was analyzed.The quercetin-producing endophytic fungi were identified by morphology and ITS sequence analysis.【Result】Nine endophytic fungi were isolated from different parts of Houttuynia cordata Thunb.and the strain YXC-G1 isolated from roots had the ability to produce quercetin.It was Acrostalagmus sp.and the content of produced quercetin in the mycelia extract was 8.296 mg/g.【Conclusion】One quercetin-producing endophytic fungus YXC-G1 was isolated from roots of Houttuynia cordata Thunb.,which could be used as an alternative strain for quercetin production.