大麻二酚对大鼠多重脑震荡炎症反应的抑制作用

Inhibition effects of cannabidiol on inflammatory response following multiple cerebral concussions in rats

目的探究大麻二酚(CBD)抑制大鼠多重脑震荡神经炎症、保护神经组织的机制。方法制备大鼠多重脑震荡模型(MCC),分别采用HE、免疫组化染色检测多重脑震荡后神经元和小胶质细胞的变化及大麻二酚的保护作用。应用Western blot检测不同脑区Iba-1(小胶质细胞标记物)及炎症因子IL-1β、TNFα的表达。结果HE染色显示,较之Sham组,大鼠MCC后神经元出现明显病理改变;CBD干预后神经元形态趋于正常,高剂量组更显著。免疫组化显示,与Sham组呈静息状态的小胶质细胞相比,MCC后数量增加呈激活状态,CBD干预后小胶质细胞向静息态恢复,高剂量组更明显。Western blot结果显示:大鼠MCC后Iba-1、IL-1β、TNFα蛋白表达显著升高(P<0.05);CBD干预可下调不同脑区Iba-1、IL-1β和TNFα的表达(P<0.05)。结论大麻二酚可减轻多重脑震荡大鼠皮质及海马的炎症反应,发挥神经保护作用。

Objective To investigate the mechanism by which cannabidiol(CBD) inhibits neuroinflammation and protects neural tissue in a rat model of multiple cerebral concussions.Methods Rat models of multiple cerebral concussions(MCC) were established,and Hematoxylin-eosin(HE) and immunohistochemical staining were used to assess changes in neurons and microglial cells after multiple cerebral concussions and the protective effects of CBD.Western blot was performed to measure the expression of microglial cell marker Iba-1 and pro-inflammatory factors IL-1β and TNFα in different brain regions.Results HE staining revealed significant pathological changes in neurons following MCC in rats compared to the Sham group.CBD intervention led to a restoration of normal neuronal morphology,with a more pronounced effect observed in the high-dose group.Immunohistochemical staining showed that microglial cells in the Sham group were in a resting state,while their numbers increased and exhibited an activated state after MCC.CBD intervention resulted in a shift of microglial cells towards a resting state,with a more significant effect observed in the high-dose group.Western blot demonstrated a significant upregulation of Iba-1,IL-1β,and TNFα protein levels in different brain regions after MCC(P<0.05).CBD intervention led to a downregulation of Iba-1,IL-1β,and TNFα expression in these brain regions(P<0.05).Conclusions Cannabidiol could alleviate inflammatory response in the cortex and hippocampus of rats with multiple cerebral concussions and exhibit neuroprotective effects.