河南孟津地区6种引起奶牛流产病原的PCR检测及分析

PCR Detection and Analysis of Six Pathogens Causing Abortion in Dairy Cows in Mengjin District of Henan

为调查孟津地区引起奶牛流产的主要病原,本实验采集该地区8个规模化奶牛养殖场共52头新鲜流产胎牛,及其母体的血液、阴道分泌物和鼻腔分泌物。对所采集样本进行新孢子虫(%N.caninum)、弓形虫(T.gondii)、胎儿三毛滴虫(T.foetus)、布氏杆菌(B.abortus%)、牛传染性鼻气管炎病毒(IBRV)和牛病毒性腹泻病毒(BVDV)6种常见流产病原的PCR和RT-PCR检测。结果显示,被检样本总感染率为44.2%(23/52),其中新孢子虫感染率最高,为32.7%(17/52);IBRV次之,为13.5%(7/52);胎儿三毛滴虫、布氏杆菌、弓形虫和BVDV感染率分别为1.9%、1.9%、1.9%和0。11.5%(6/52)的被检样本存在混合感染,其中以新孢子虫+IBRV混合感染为主(50%,3/6)。结合被检流产奶牛的临床特征及饲养管理等因素,推断新孢子虫是导致该地区奶牛流产的主要病原。

To investigate the main pathogens causing abortion in dairy cows in Mengjin area,this study collected samples from 52 fresh aborted fetuses and their maternal blood,vaginal secretions,and nasal secretions from eight large-scale dairy farms in the region.The collected samples were tested for six common abortion-causing pathogens,including Neospora caninum%(N.caninum)%,Toxoplasma gondii%(T.gondii),%Tritrichomonas foetus%(T.foetus%),Brucella abortus%(B.abortus),%Bovine Herpesvirus 1(BoHV-1),and Bovine viral diarrhea virus(BVDV)using PCR and RT-PCR techniques.The results showed that the overall infection rate of the tested samples was 44.2%(23/52),with Neospora caninum having the highest infection rate at 32.7%(17/52);followed by BoHV-1 at 13.5%(7/52);while Tritrichomonas foetus,Brucella abortus,Toxoplasma gondii,and BVDV had respective infection rates of 1.9%,1.9%,1.9%,and 0.Mixed infections were found in 11.5%(6/52)of the tested samples,with Neospora caninum+BoHV-1 being the predominant combination(50%,3/6).Considering the clinical characteristics of the aborted dairy cows and factors related to feeding management,it is inferred that Neospora caninum is the primary pathogen causing abortions in this region.