健脾温肾方对荷肉瘤小鼠腹水模型免疫细胞相关因子影响研究

Effects of Jianpi Wenshen Formula on Immune Cell-related Factors in the Ascites Model of Sarcoma-bearing Mice

目的:探讨健脾温肾方对荷S180肉瘤小鼠腹水模型免疫细胞相关因子水平影响。方法:5~6周龄的C57小鼠60只,随机分为模型组、顺铂组、顺铂+低剂量组以及顺铂+高剂量组,每组15只。制备荷S180肉瘤小鼠腹水模型。顺铂组给予0.2 mg/kg顺铂,每周第1天、第4天、第7天给药,给药4周。顺铂+低剂量组在顺铂组基础上灌胃9.75 g/kg健脾温肾方,1次/d,共4周。顺铂+高剂量组在顺铂组基础上灌胃19.5 g/kg健脾温肾方,1次/d,共4周。模型组给予等剂量生理盐水腹腔注射,每周第1天、第4天、第7天给药,给药4周。比较各组小鼠瘤重和抑瘤率、腹水量、红细胞和肿瘤细胞计数、癌胚抗原(CEA)、免疫细胞相关因子变化;并记录小鼠生存时间。结果:顺铂+低剂量组和顺铂+高剂量组抑瘤率高于顺铂组(P<0.05),且呈剂量依赖性。与模型组比较,顺铂组、顺铂+低剂量组以及顺铂+高剂量组瘤重、腹水量、红细胞计数和肿瘤细胞计数减少,CEA、血管内皮生长因子(VEGF)、白细胞介素-4(IL-4)水平降低而γ干扰素(IFN-γ)水平升高,生存时间延长(P<0.05);顺铂+低剂量组和顺铂+高剂量组瘤重、腹水量、红细胞计数和肿瘤细胞计数低于顺铂组,CEA、VEGF、IL-4水平低于顺铂组而IFN-γ高于顺铂组,生存时间长于顺铂组(P<0.05),且呈剂量依赖性。结论:健脾温肾方对荷S180肉瘤小鼠腹水模型抑瘤率显著,可减少腹水量,且可下调VEGF和IL-4水平及上调IFN-γ水平。

Objective:To explore the effects of Jianpi Wenshen Formula(JPWSF) on the levels of immune cell-related factors in the ascites model of sarcoma-180(S180)-bearing mice.Methods:Sixty C57 mice of 5 to 6 weeks old were randomized into a model group,a cisplatin group,a cisplatin+low-dose(9.75 g/kg) JPWSF group,and a cisplatin+high-dose(19.5 g/kg) JPWSF group,with 15 mice in each group.A ascites model of S180-bearing mice was established.The mice were administrated with cisplatin at a dose of 0.2 mg/kg by gavage on days 1,4,and 7 of each week for four weeks.JPWSF was administrated at corresponding doses once a day for a total of 4 weeks.The model group received intraperitoneal injection of an equal dose of normal saline on days 1,4,and 7 of each week for 4 weeks.The tumor weight,tumor inhibition rate,ascites liquid volume,red blood cell and tumor cell counts,carcino-embryonic antigen(CEA) level,and levels of immune cell-related factor were compared between groups.The survival time of the mice was recorded.Results:Compared with the cisplatin group,the additional administration of JPWSF increased the tumor inhibition rate in a dose-dependent manner(P<0.05).Compared with the model group,the cisplatin,cisplatin+low-dose JPWSF,and cisplatin+high-dose JPWSF groups showed decreased tumor weight,ascites liquid volume,red blood cell count,and tumor cell count,lowered levels of CEA,vascular endothelial growth factor(VEGF),and interleukin-4(IL-4),elevated level of interferon-gamma(IFN-γ),and prolonged the survival time(P<0.05).Compared with the cisplatin group,the additional administration of JPWSF reduced the tumor weight,ascites liquid volume,red blood cell count,and tumor cell count,lowered the levels of CEA,VEGF,and IL-4,elevated the IFN-γ level,and prolonged the survival time in a dose-dependent manner(P<0.05).Conclusion:JPWSF significantly inhibited the tumor,reduced the ascites liquid,lowered the VEGF and IL-4 levels,and elevated the IFN-γ level in the ascites model of S180-bearing mice.