黄芪多糖上调γH2AX的表达提高宫颈癌细胞放疗敏感性的分析

Astragalus Polysaccharide Improve the Sensitivity of Radiotherapy in Cervical Cells by Promoting the Expression ofγH2AX

目的研究黄芪多糖(APS)对宫颈癌Siha细胞增殖能力的影响,观察放疗后磷酸化组蛋白H2AX(γH2AX)的表达情况。方法体外培养Siha细胞,将其分为两组即放疗组和APS联合放疗组。应用CCK8法检测不同浓度APS对Siha细胞增殖活性的影响;Western blotting检测放疗4 h后两组γH2AX蛋白的表达水平。结果APS浓度越高,细胞的增殖活力越弱;APS作用时间越长,细胞增殖能力越弱(P<0.01);放疗后,APS联合放疗组γH2AX表达水平高于放疗组(P=0.031)。结论APS可以抑制Siha细胞增殖,促进γH2AX表达,从而提高放疗的敏感性。

Objective To study the effect of Astragalus polysaccharide(APS)on the proliferation of Siha cells with cervical cancer and the expression of phosphorylated histone H2AX(γH2AX)after radiotherapy.Methods Siha cells were cultured in vitro,and the 2 groups were divided into radiotherapy group and Astragalus polysaccharide combined with radiotherapy group.Cell proliferation was analyzed by CCK 8,andγH2AX expression level after radiotherapy was measured by Western blotting.Results The higher concentration of APS led to weaker proliferation of Siha cells;With longer period exposed of APS,the less proliferation compared with the radiotherapy group;The expression level ofγH2AX in the APS combined with radiotherapy group suggested was higher than that in the radiotherapy group at 4 hours after radiotherapy.Conclusion APS can inhibit the proliferation of Siha cells,and promote the expression ofγH2AX,maybe can improve the sensitivity of radiotherapy.