乳痛愈药膜外敷对乳腺增生症模型大鼠的作用

Effect of external application of Breast Pain Healing Film on rats with mammary gland hyperplasia

目的观察乳痛愈药膜外敷对乳腺增生症模型大鼠的作用。方法将55只大鼠按照随机数字表法分为5组,即空白组、模型组、三苯氧胺软膏组、散结乳癖贴膏组和乳痛愈药膜组,每组11只。除空白组外,其余各组建立乳腺增生模型。造模成功后,空白组、模型组大鼠于第2对乳腺部位外用空白赋形剂0.1 g/只,三苯氧胺软膏组于第2对乳腺部位外用三苯氧胺软膏0.1 g/只,散结乳癖贴膏组予散结乳癖贴膏0.4 g/只(相当于原散结乳癖贴膏0.1 g),乳痛愈药膜组予乳痛愈药膜0.8 g/只(相当于生药0.8 g)。观察各组大鼠乳头直径、高度变化;比较各组大鼠脾脏指数、胸腺指数;比较各组大鼠血清雌二醇(E2)、孕酮(P)、催乳素(PRL)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平变化;观察各组大鼠乳腺组织病理形态;比较各组大鼠乳腺组织雌激素受体α(ERα)、ERβ和孕激素受体(PR)表达。结果与空白组比较,模型组乳头直径、乳头高度均增加(P<0.05);与模型组比较,三苯氧胺软膏组、散结乳癖贴膏组、乳痛愈药膜组乳头直径、乳头高度均减小(P<0.05);三苯氧胺软膏组、散结乳癖贴膏组、乳痛愈药膜组乳头直径、乳头高度比较差异均无统计学意义(P>0.05)。与空白组比较,模型组脾脏指数、胸腺指数均降低(P<0.05);与模型组比较,各给药组大鼠脾脏指数、胸腺指数均升高(P<0.05);三苯氧胺软膏组、散结乳癖贴膏组、乳痛愈药膜组大鼠脾脏指数、胸腺指数比较差异无统计学意义(P>0.05)。与空白组比较,模型组血清E2、PRL水平升高(P<0.05),P水平降低(P<0.05);与模型组比较,各给药组血清E2、PRL水平降低(P<0.05),P水平升高(P<0.05);散结乳癖贴膏组P水平高于三苯氧胺软膏组(P<0.05)。与空白组比较,模型组MDA水平升高(P<0.05),SOD水平降低(P<0.05);与模型组比较,各给药组血清MDA水平均降低(P<0.05),SOD水平均升高(P<0.05);与三苯氧胺软膏组比较,散结乳癖贴膏组、乳痛愈药膜组血清MDA水平均降低(P<0.05),SOD水平均升高(P<0.05)。与空白组比较,模型组大鼠乳腺组织ERα、ERβ、PR表达均升高(P<0.05);与模型组比较,各给药组大鼠乳腺组织ERα、ERβ表达均降低(P<0.05);各给药组大鼠乳腺组织ERα、ERβ、PR表达比较差异均无统计学意义(P>0.05)。结论乳痛愈药膜能改善乳腺增生症模型大鼠乳腺组织增生程度,调节血清性激素水平,调节自由基代谢和乳腺组织中雌激素受体(ERα、ERβ)表达,减弱雌激素(E2)的生物学效应。

Objective To observe the effect of external application of Breast Pain Healing Film(BPHF)on rats with mammary gland hyperplasia.Methods A total of 55 rats were randomly divided into control group,model group,tamoxifen ointment group,SanjieRupi Plaster(SJRPP)group and BPHF group,with 11 rats per group.Rats in the latter four groups were subjected to the modeling of mammary gland hyperplasia.After successful modeling,rats in the control group and model group were externally applied with 0.1 g blank vehicle per rat on the second pair of mammary glands.Those in the tamoxifen ointment group,SJRPP group and BPHF group were externally applied with 0.1 g tamoxifen ointment,0.4 g SJRPP(equivalent to 0.1g of the original SanjieRupi Patch)and 0.8 g BPHF(equivalent to 0.8 g of crude drug)per rat on the second pair of mammary glands,respectively.Changes in the nipple appearance of rats were observed.Spleen index and thymus index were recorded.Serum estradiol(E 2),progesterone(P),prolactin(PRL),malondialdehyde(MDA)and superoxide dismutase(SOD)levels were measured.Pathological morphology of the breast tissue was visualized,and expressions of estrogen receptorα(ERα),ERβand progesterone receptor(PR)in the breast tissue were detected.Results Compared with those of the control group,the nipple diameter and height of the model group significantly increased(P<0.05).Compared with those of the model group,the nipple diameter and height of the tamoxifen ointment group,SJRPP group and BPHF group significantly decreased(P<0.05),which were comparable among the latter three groups(P>0.05).Compared with those of the control group,the spleen index and thymus index of the model group significantly decreased(P<0.05).Compared with those of the model group,the spleen index and thymus index of the tamoxifen ointment group,SJRPP group and BPHF group significantly increased(P<0.05),which were comparable among the latter three groups(P>0.05).Compared with those of the control group,rats in the model group presented significantly higher E 2 and PRL,and lower P(both P<0.05).Compared with those of model group,rats in the tamoxifen ointment group,SJRPP group and BPHF group presented significantly lower E 2 and PRL,and higher P(both P<0.05).P level was significantly higher in SJRPP group than that of tamoxifen ointment group(P<0.05).Compared with those of the control group,rats in the model group presented significantly higher MDA and lower SOD(both P<0.05).Compared with those of model group,rats in the tamoxifen ointment group,SJRPP group and BPHF group presented significantly lower MDA and higher SOD(both P<0.05).Compared with those of tamoxifen ointment group,rats in the SJRPP group and BPHF group presented significantly lower MDA and higher SOD(both P<0.05).Compared with those of the control group,rats in the model group presented significantly higher ERα,ERβand PR in the mammary gland tissue(P<0.05).Compared with those of model group,rats in the tamoxifen ointment group,SJRPP group and BPHF group presented significantly lower ERα,ERβand PR in the mammary gland tissue(P<0.05),which were comparable among the latter three groups(P>0.05).Conclusion BPHF can improve the hyperplasia of mammary gland tissue in rats,presenting the biological effect on regulating serum sex hormone,free radical metabolism and estrogen receptors(ERα,ERβ)in mammary gland tissue,and weakening the secretion of E 2.