闭鞘姜7个部位α-葡萄糖苷酶抑制活性及其乙醚提取物的GC-MS分析

α-Glucosidase Inhibitory Activities of Seven Parts from Costus speciosus and GC-MS Analyses of Their Ether Extracts

用乙醚和乙醇对闭鞘姜7个部位进行提取后,用pNPG(4-硝基苯基-β-D-吡喃葡萄糖苷)法测试α-葡萄糖苷酶体外抑制活性,并通过气相色谱-质谱(GC-MS)联用方法分析测定其乙醚相中的化学成分。共检出化学成分125个,通过峰面积归一化法确定各化学成分的相对含量,结果显示,闭鞘姜7个部位化学成分差异较大。活性测试结果表明:花瓣、花萼和须根的乙醚提取物的酶抑制率均超过70%,茎的乙醚提取物和根茎的乙醇提取物的酶抑制率在50%左右。因此,闭鞘姜不同部位挥发性成分差异较大,花瓣、花萼和须根的乙醚提取物具有相对较强的α-葡萄糖苷酶抑制活性,可为闭鞘姜全株的资源利用提供参考。

Seven different parts of fresh Costus speciosus were extracted by ultrasonic extraction with ether and alcohol respectively,and all the samples were testedα-glucosidase inhibitory effect in vitro by pNPG(4-nitrophenyl-beta-d-glucopyranoside)assay.The chemical components of ether extract were analyzed by gas chromatography-mass spectrometry(GC-MS).A total of 125 chemical constituents were detected by GC-MS whose relative contents were determined by peak area normalization method.Results showed that the chemical constituents in each extract significantly differed from others.The bioactivity results showed that the ether extracts of petal,calyxe,and fibrous root presented inhibition ofα-glucosidase rate up to 70%,while the ether extract of stem and the alcohol extract of rhizome had the inhibition rate around 50%.In summary,the volatile components in different extracts showed difference,and the ether extracts of petal,calyxe and fibrous root showed relatively strong inhibitory activity ofα-glucosidase in vitro,which can provide a reference for the utilization of the whole plant resource of Costus speciosus.